| 感染NDV后鸡腺胃中P物质和VIP的分布及动态观察 |
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| 引用本文： | 尚云连,佘锐萍,杨玉荣,刘天龙.感染NDV后鸡腺胃中P物质和VIP的分布及动态观察[J].科学技术与工程,2006,6(13):1785-17881797. |
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| 作者姓名： | 尚云连 佘锐萍 杨玉荣 刘天龙 |
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| 作者单位： | 中国农业大学,北京,100094 |
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| 基金项目： | 国家自然科学基金（30270995,30471301）资助 |
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| 摘 要： | 70只35日龄SPF鸡随机分成对照组和攻毒组.攻毒组经腹腔接种鸡新城疫病毒后,分别取腺胃,并用免疫组织化学方法检测组织中NDV、P物质和VIP的分布及动态变化.结果表明：P物质和VIP在两组鸡的腺胃中都有存在,且三种物质的表达量呈现正相关性,其含量在感染后有增加,证明了P物质和VIP在感染NDV后有调节动物防御系统的作用.
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| 关 键 词： | 新城疫（ND） 免疫组织化学 腺胃 P物质 血管活性肠肽（VIP） |
| 文章编号： | 1671-1815（2006）13-1785-05 |
| 收稿时间： | 2006-03-07 |
| 修稿时间： | 2006年3月7日 |
The Alter Expression and Distribution of NDV, Substance P & VIP in the Stomachus Glandulars of Chicken Infected with NDV |
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| SHANG Yunlian,SHE Ruiping,YANG Yurong,LIU Tianlong.The Alter Expression and Distribution of NDV, Substance P & VIP in the Stomachus Glandulars of Chicken Infected with NDV[J].Science Technology and Engineering,2006,6(13):1785-17881797. |
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| Authors: | SHANG Yunlian SHE Ruiping YANG Yurong LIU Tianlong |
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| Abstract: | Seventy 35-day-old chickens were randomly divided into two groups. 35 chickens were used for infection group and the others were served as control. The animals were taken stomachus glandularis after infected with NDV, SP and measured the NDV, VIP and SP with immunohistochemical method. The results indicated that the expression of NDV,SP and VIP in stomachus glandulars increased after the chickens infected with NDV. There were correlated relations between the expression of SP and VIP and the expression of NDV in some extent. |
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| Keywords: | Newcastle disease immunohistochemical stomachus glandulars SP VIP |
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