汉滩病毒S基因0.7kb片段与M基因G2片段不同拼接方式嵌合基因原核表达产物的初步比较

比较汉滩病毒S、M基因部分片段的嵌合基因不同拼接方式表达产物的活性。构建了嵌合基因原核表达载体pGEX-4T-1-S0.7G2,并与已构建的pGEX-4T-1-G2S0.7的诱导表达产物进行了比较。结果表明,融合蛋白同时保持亲本蛋白的结合活性,且前者表达产物的结合活性始终比后者低。研究表明,嵌合基因的不同拼接方式对融合蛋白的活性可能有一定的影响。

Preliminary Studies on the Comparison of Prokaryotic Expression Products of Different Chimeric Genes Containing Hantaan Virus S and M Segments

To express the chimeric gene containing Hantaan virus S and M segments in prokaryotic expression system and compare the activities of the chimeric genes constructed by different splicing ways. The prokaryotic expression vector pGEX-4T-1-S0.7G2 was constructed. After inducing expressed the fused protein, Western-blot and ELISA methods were used to compared the activities of N0.7G2 with G2N0.7. The results showed that the activities of N0.7G2 was lower than that of G2N0.7. It is suggested that the splicing ways of chimeric genes may affect the activities of fused proteins expressed by the chimeric genes.