| 体外APC/C活性体系的建立及其影响因素的研究 |
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| 引用本文： | 高彦飞,李腾,常艳,王玉博,穆蕊,陈亮,甄诚,韩秋影,于鸣,巩伟丽,张维娜,李慧艳.体外APC/C活性体系的建立及其影响因素的研究[J].科学技术与工程,2011,11(21). |
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| 作者姓名： | 高彦飞 李腾 常艳 王玉博 穆蕊 陈亮 甄诚 韩秋影 于鸣 巩伟丽 张维娜 李慧艳 |
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| 作者单位： | 1. 国家生物医学分析中心,北京,100850
2. 军事民学科学院基础医学研究所细胞免疫学教研室,北京,100850 |
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| 基金项目： | 国家自然科学基金(30871234)资助 |
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| 摘 要： | 为获得具有细胞周期后期促进复合物(APC/C)活性的细胞裂解液并建立APC/C体外活性检测体系,以其底物Cyc-lin B及Securin的降解为检测指标,分别从细胞阻滞于有丝分裂期的时间,UBCH10(APC/C的E2酶)的浓度以及裂解液浓度等几个方面研究了各种处理因素对APC/C活性的影响,并且用体外翻译底物验证了其活性及相关结论。此项工作为进一步探索APC/C相关分子事件奠定了基础。
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| 关 键 词： | 后期促进复合物 Cyclin B Securin UBCH10 活性 |
| 收稿时间： | 4/8/2011 12:00:00 AM |
| 修稿时间： | 4/8/2011 12:00:00 AM |
Preparation of extracts with APC/C activity and analysis of its influencing factors |
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| Gao Yanfei,Li Teng,Chang Yan,Wang Yubo,Mu Rui,Chen Liang,Zhen Cheng,Han qiuying,Yu Ming,Gong Weili,Zhang Weina and Li Huiyan.Preparation of extracts with APC/C activity and analysis of its influencing factors[J].Science Technology and Engineering,2011,11(21). |
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| Authors: | Gao Yanfei Li Teng Chang Yan Wang Yubo Mu Rui Chen Liang Zhen Cheng Han qiuying Yu Ming Gong Weili Zhang Weina and Li Huiyan |
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| Institution: | GAO Yan-fei1,LI Teng1,CHANG Yan1,WANG Yu-bo1,MU Rui1,CHEN Liang1,ZHEN Cheng1,HAN Qiu-ying1,YU Ming2,GONG Wei-li1,ZHANG Wei-na1,LI Hui-yan1 (National Center of Biomedical Analasis,Beijing 100850,P.R.China,Department of Molecular Immunology1,Institute of Basic Medical Sciences,Academy of Military Medical Sciences,P.R.China) |
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| Abstract: | To prepare the cell extracts with the APC/C activity and analysis of its influencing factors,the degradation of APC/C's substrates Cyclin B and Securin was used as a marker.Several assays were done to analysis its influencing factors,including the duration of cells arrest in mitosis,the activity of extracts,the presence of its E2 enzyme,UBCH10,and the concentration of extracts.The similar result was obtained using the in vitro translated substrates.Taken together,these assays were the basement to further st... |
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| Keywords: | APC/C Cyclin B Securin UBCH10 activity |
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