2-巯基苯并噻唑衍生物类小分子抑制剂对人结直肠癌细胞SW620的生长抑制作用

拟研究2-巯基苯并噻唑衍生物类小分子抑制剂(SKLB-163)对结直肠癌细胞SW620增殖及凋亡的影响;并探讨可能的作用机制。使用流式细胞术检测药物处理后肿瘤细胞凋亡的情况;利用MTT实验检测细胞增殖情况。在裸鼠人结直肠癌细胞SW620移植瘤模型中评估药物灌胃给药时能否抑制肿瘤生长。流式细胞术和MTT表明,SKLB-163能够促进SW620细胞凋亡,且能够显著抑制SW620细胞的增殖。动物实验表明,口服灌胃给药SKLB-163(200 mg/kg)能够显著抑制裸鼠人结直肠癌细胞SW620移植模型肿瘤的生长。总之,SKLB-163具有较好的抗肿瘤活性;为进一步发展新型2-巯基苯并噻唑类抗肿瘤药物提供了依据。

Anti-tumor study of novel benzothiazole-2-thiol derivatives

This paper is to study antitumor activity of SKLB-163 in vitro and in vivo and reveal the possible molecular mechanism. Flow cytometry and MTT assay were used to detect cell apoptosis and proliferation induced by SKLB-163. The anti-tumor effects was investigated in SW620 mice model. Flow cytometry and MTT assay showed compound SKLB-163 could induce cell apoptosis and inhibit SW620 cell proliferation. The anti-tumor exprements in vivo showed compound SKLB-163 could significantly inhibit growth of SW620 tumor model. In conclusion, SKLB-163 showed a good anti-cancer activity. These findings will contribute to development of novel benzothiazole-2-thiol anti-cancer drugs.