| 正交试验优化马面 Navodon septentrionalos 卵巢磷脂的提取条件 |
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| 引用本文： | 杨文鸽,娄永江,桑卫国.正交试验优化马面 Navodon septentrionalos 卵巢磷脂的提取条件[J].浙江海洋学院学报(自然科学版),2001,20(1). |
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| 作者姓名： | 杨文鸽 娄永江 桑卫国 |
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| 作者单位： | 宁波大学生命科学与生物工程学院, |
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| 摘 要： | 用正交试验探讨了从马面卵巢中提取磷脂的方法，得到最佳工艺条件：提取剂用量为样品重 14倍 , 提取剂中氯仿与甲醇体积比 2∶ 1, 抽提时间 4 h，洗涤分层时加滤液体积一半的 1 ％ NaCl溶液 ,其中前三个因素对实验结果有显著影响。
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| 关 键 词： | 马面卵巢 磷脂 正交试验 提取 |
Application of Orthogonal Trials to Phospholipids Extraction of Navodon septentrionalos(Gü nther) Ovary |
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| YANG Wen-ge,LOU Yong-jiang,SANG Wei-guo.Application of Orthogonal Trials to Phospholipids Extraction of Navodon septentrionalos(Gü nther) Ovary[J].Journal of Zhejiang Ocean University(Natural Science Edition),2001,20(1). |
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| Authors: | YANG Wen-ge LOU Yong-jiang SANG Wei-guo |
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| Abstract: | The method of phospholipids extraction from Navodon septentrionalos(Gü nther) ovary was discussed by orthogonal trials .The results indicated that the optimum condition of extraction is as follows: extractant(ml):sample(g)=14:1, chloroform(ml):methanol(ml)=2:1, four hours'extraction, 1％ NaCl(ml):extratum(ml)=1:2.In addition， the results is affected apparently by the three factors except the volume of 1％ NaCl. |
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