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菲律宾蛤仔糖蛋白提取及体外抗肿瘤活性研究
引用本文:郁迪,杨永芳,王加斌,杨最素,黄芳芳,郑玉寅,李荣,丁国芳.菲律宾蛤仔糖蛋白提取及体外抗肿瘤活性研究[J].浙江海洋学院学报(自然科学版),2011,30(4):336-339.
作者姓名:郁迪  杨永芳  王加斌  杨最素  黄芳芳  郑玉寅  李荣  丁国芳
作者单位:1. 浙江海洋学院食品与药学学院,浙江舟山,316004
2. 浙江海力生制药有限公司,浙江舟山,316021
基金项目:浙江省自然科学基金项目(Y3100129); 浙江省教育厅研究生创新科研项目(YK2008072); 浙江省新苗人才计划项目(2008R40G2110028); 舟山市科技计划海洋专项(092016)
摘    要:以菲律宾蛤仔肉为研究对象,水提醇沉,Sevag法除蛋白,DEAE-Sepharose FF阴离子交换层析柱分离纯化,Sephadex G-25凝胶柱除盐,得到1种白色组分,红外显示该组分具有糖蛋白的一般吸收特征。将该糖蛋白作用于前列腺癌细胞DU-145进行体外药物敏感性实验,结果显示,0.1 mol/L氯化钠溶液的洗脱峰组分对DU-145细胞具有明显增殖抑制作用,最适作用浓度约为25 mg/mL,表明菲律宾蛤仔具有潜在的抗肿瘤作用。

关 键 词:菲律宾蛤仔  糖蛋白  抗肿瘤活性

Study of Glycoprotein Extracted and Purified From Ruditapes philippinarum Inhibits the Growth of Tumor Cell in Vitro
YU Di,YANG Yong-fang,WANG jia-bin,et al.Study of Glycoprotein Extracted and Purified From Ruditapes philippinarum Inhibits the Growth of Tumor Cell in Vitro[J].Journal of Zhejiang Ocean University(Natural Science Edition),2011,30(4):336-339.
Authors:YU Di  YANG Yong-fang  WANG jia-bin  
Institution:YU Di1,YANG Yong-fang1,WANG jia-bin2,et al(1.School of Food and Pharmacy of Zhejiang Ocean University,Zhoushan 316004,2.Hailisheng Pharmaceutical Co Ltd,Zhoushan 316021,China)
Abstract:The glycoprotein are extracted and purified from Philippine clam meat by ethanol precipitation,protein filtration with Sevag reagent,DEAE Sepharose FF and Sephadex G-25 column chromatography.The glycoprotein inhibits the growth of DU-145 cells remarkably at a low level dose,and its optimal concentration is 25 mg/mL.The results suggest that Philippine clam meat probably contains potent anti-tumor groups,which is extracted from the glycoprotein.
Keywords:Ruditapes philippinarum  glycoprotein  anti-tumor activity  
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