| 具有抑菌活性的海藻多酚联合提取工艺优化研究 |
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| 引用本文： | 杨会成,;郑斌,;郝云彬,;相兴伟,;周宇芳,;廖妙飞,;李瑞雪.具有抑菌活性的海藻多酚联合提取工艺优化研究[J].浙江海洋学院学报(自然科学版),2014(2):147-153. |
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| 作者姓名： | 杨会成 ;郑斌 ;郝云彬 ;相兴伟 ;周宇芳 ;廖妙飞 ;李瑞雪 |
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| 作者单位： | [1]浙江省海洋开发研究院,浙江舟山316021; [2]中国海洋大学食品科学与工程学院,山东青岛266003; [3]舟山出入境检验检疫局,浙江舟山316100 |
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| 基金项目： | 国家海洋局海洋公益性行业科研专项（2011-2-2-07-3）;浙江省重点科技创新团队项目（2011R09031-13）;国家科技支撑计划项目子课题（2012BAD29806） |
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| 摘 要： | 采用超声波、微波联合浸提方式提取海藻多酚,通过正交实验确立了多酚提取工艺条件,并以平板打孔抑制法测定了海藻多酚粗提物对7种细菌和4种真菌的抑菌活性。结果表明:采用料液比(匀浆:乙醇,g/mL)1:7,乙醇浓度85%,浸提温度70℃,浸提次数2次,浸提时间4 h,提取效果最佳,海带多酚提取率为2.08%。海藻多酚对供试的2种革兰氏阳性菌和5种革兰氏阴性菌都有一定程度的抑制活性,量效关系均呈剂量依赖型,最小抑菌浓度为10~40μg/mL。其中对大肠杆菌、绿脓杆菌抑制效果较明显,对青霉、白色念珠菌亦有抑制作用。
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| 关 键 词： | 海藻 多酚 提取 抑菌 |
Study on the Combined Extraction Technologyof Algae Polyphenols with Antibacterial Activity |
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| Institution: | YANG Hui-cheng, ZHENG Bin, HAO Yun-bin, et al (1. Zhejiang Marine Development Research Institute, Zhoushan 316100; 2. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China) |
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| Abstract: | Method of microwave and ultrasonic joint extraction of algae polyphenols was established by orthogonal experiment, and meanwhile antimicrobial activity of polyphenols was carried out in seven kinds of bacteria and four fungi by flat punch. The best process was solid-liquid ratio （homogenized：ethanol, g/mL） 1：7, 85% ethanol concentration, temperature 70 ℃, extraction 2 times, extraction 4 h, kelp polyphenols extraction rate 2.08%. Polyphenols had antimicrobial effects on 2 Gram-positive bacteria and five Gram-negative bacteri-a in a dose-dependent effect relationship type. The minimum inhibitory concentration of 10-40 μg/mL, which showed obvious inhibitory effect on E. coli, Pseudomonas aeruginosa, Penicillium, Candida albicans also inhib-ited. |
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| Keywords: | algae polyphenols extraction antibacterial |
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