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| 松材线虫快速检测试剂盒研制 | |
| 引用本文： | 陈凤毛,叶建仁,吴小芹,汤坚.松材线虫快速检测试剂盒研制[J].南京林业大学学报(自然科学版),2007,31(4):133-135. |
| 作者姓名： | 陈凤毛 叶建仁 吴小芹 汤坚 |
| 作者单位： | 1. 南京林业大学森林资源与环境学院,江苏,南京,210037;安徽省林业厅,安徽,合肥,230031 2. 南京林业大学森林资源与环境学院,江苏,南京,210037 3. 安徽省林业厅,安徽,合肥,230031 |
| 基金项目： | 安徽省林业厅与南京林业大学合作研究项目 , 江苏省博士后基金 |
| 摘 要： | 采用引物对K09F2/R对枯死松树内分离的松材线虫、拟松材线虫及其他线虫进行检测,结果表明,所有松材线虫株系均扩增出一条340 bp的清晰、明亮的条带.而拟松材线虫、其他线虫均无扩增产物.利用引物对K09F2/R构建了松材线虫检测试剂盒.采用该试剂盒可使整个检测过程不超过2.5 h;该试剂盒检测灵敏度为1/7 条线虫,达到了对幼虫成功鉴定的目的,且检测结果便于判读,检测费用较低. |
| 关 键 词： | 松材线虫 快速检测 试剂盒 拟松材线虫 快速 检测试剂盒 Detection Quick 检测费用 判读 幼虫 检测灵敏度 检测过程 利用 扩增产物 株系 结果 分离 松树 |
| 文章编号： | 1000-2006（2007）04-0133-03 |
| 修稿时间： | 2006-09-272007-04-16 |
Development of Quick Detection Kit for Bursaphelenchus xylophilus |
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| CHEN Feng-mao,YE Jian-ren,WU Xiao-qin,TANG Jian.Development of Quick Detection Kit for Bursaphelenchus xylophilus[J].Journal of Nanjing Forestry University(Natural Sciences ),2007,31(4):133-135. | |
| Authors: | CHEN Feng-mao YE Jian-ren WU Xiao-qin TANG Jian |
| Institution: | 1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037 ,China; 2. Anhui Forestry Department, Hefei 230031 ,China |
| Abstract: | Bursaphelenchus xylophilus, B. rnucronatus and the other nematodes separated from dead pine .were detected by using primer K09F2/R. The results indicated that the PCR product of all B. xylophilus isolates had a clear and bright fragment about 340bp. But B. mucronatus and other nematodes had not any fragments. So pairwise K09F2/R was used for constructing identification kit of B. xylophilus. It took a short time to identify nematodes by adopting this kit. The time of total detection process was within two and half hours. Besides, the sensitivity of this kit reached to 1/7 of a nematode. The detection result is easy to estimate, and the cost of detection is quite low. This kit is a rapid, reliable and convenient means for detecting B. xylophilus. |
| Keywords: | Bursaphelenchus xylophilus Quick detection Kit |
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