| 银杏、板栗不同组织DNA的提取 |
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| 引用本文： | 沈永宝,施季森.银杏、板栗不同组织DNA的提取[J].南京林业大学学报(自然科学版),2001,25(6):80-82. |
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| 作者姓名： | 沈永宝 施季森 |
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| 作者单位： | 国家林业局南方林木种子检验中心,江苏南京,210037 |
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| 基金项目： | 国家林业局97’重点课题“利用RAPD、AFLPDNA分子标记技术构建银杏、油茶、板栗指纹图谱的研究”部分内容 |
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| 摘 要： | 利用CTAB方法提取银杏、板栗的冬芽、嫩叶、半木质化韧皮部、老枝韧皮部和成熟叶子的DNA。结果表明，除成熟叶子外，其它4种组织的DN较容易提取，且DNA的纯度较高，A260/A280值都在1.8以上，每100mg材料的产量为22～50μg。清晰明亮的PCR产物也表明所提取的DNA可满足DNA分子标记的要求。
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| 关 键 词： | DNA提取 银杏 板栗 CTAB法 组织器官 DNA分子标记 |
| 文章编号： | 1000-2006(2001)06-0080-03 |
| 修稿时间： | 2001年6月4日 |
DNA Extraction from Different Tissues of Ginkgo and Chinese Chestnut |
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| SHEN Yong-bao.SHI Ji-sen.DNA Extraction from Different Tissues of Ginkgo and Chinese Chestnut[J].Journal of Nanjing Forestry University(Natural Sciences ),2001,25(6):80-82. |
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| Authors: | SHEN Yong-baoSHI Ji-sen |
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| Abstract: | This paper shows that the genomic DNA from different tissues of Ginkgo and Chinese chest- nut was extracted by CTAB method. The results indicate that except for mature leaf, other tissues, uch as dormant bud, young leaf, phloem and half-lignous phloem, can be used to extract DNA easily and successfully. In addition,the value of A260/A280 for each DNA samples above mentioned is over 1. 8,and the quantity is over 22~50 g per 100 mg. Also,clear and bright products of RAPD reveal that the DNA may meet needs of DNA molecular markers. |
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| Keywords: | DNA extraction Ginkgo Chinese chestnut |
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