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植物冰冻切片技术的改进
引用本文:刘剑锋,程云清,阎秀峰,周革,王淑范.植物冰冻切片技术的改进[J].南京林业大学学报(自然科学版),2006,30(3):128-130.
作者姓名:刘剑锋  程云清  阎秀峰  周革  王淑范
作者单位:1. 吉林师范大学生命科学学院,吉林,四平,136000
2. 东北林业大学生命科学学院,黑龙江,哈尔滨,150040
3. 四平市园林管理处,吉林,四平,136000
摘    要:以五味子叶柄、叶片、根和茎为试材进行冰冻切片,过程简单有效且所得图版清晰,在1天内即可获得高质量图片。其关键步骤是选择合适的甘油浓度、切片时的温度及展片技术。

关 键 词:冰冻切片  改进技术  五味子
文章编号:1000-2006(2006)03-0128-03
收稿时间:2005-06-08
修稿时间:2005-12-11

Improvement of Plant Cryo-sectioning Technique
LIU Jian-feng,CHENG Yun-qing,YAN Xiu-feng,ZHOU Ge,WANG Shu-fan.Improvement of Plant Cryo-sectioning Technique[J].Journal of Nanjing Forestry University(Natural Sciences ),2006,30(3):128-130.
Authors:LIU Jian-feng  CHENG Yun-qing  YAN Xiu-feng  ZHOU Ge  WANG Shu-fan
Institution:1. School of Life Sciences Jilin Normal University, Siping 136000,China; 2. College of Life Sciences Northeast Forestry University, Harbin 150040, China ; 3. Landscape Office of Siping Jilin Province, Siping 136000, China
Abstract:It was a time consuming process if technique of paraffin section was used to observe plant specimen because fussy processes such as dehydrating,infiltrating and embedding were needed before being observed.Cryo-sectioning technique was used to observe leafstalk,leaf,stem and root structure of Schisandra chinensis(Turez.)Ball..The operating process was simple and efficient.Clear image could be acquired within one day.The crucial step were to select a proper concentration of glycerol,a suitable temperature of freezing and an appropriate way of section-flatting.
Keywords:Cryo-sectioning  Technique improvement  Schisandra chinensis(Turez  )Ball  
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