侧柏古树扦插试验及插穗营养物质变化

【目的】 提高侧柏古树的嫩枝扦插生根率,建立侧柏古树扦插繁殖技术体系。【方法】 采用3种植物生长调节剂ABT1,NAA和IBA+NAA(等质量比)]的3组质量浓度(200~600,1 000~3 000和4 000~10 000 mg/L)处理采自5、100、300、700 a侧柏的插穗,对插穗生根进程、生根性状(生根率、生根数量、最长根长)、生根进程中营养物质(可溶性淀粉、可溶性糖)含量进行动态测定。【结果】 通过观察发现:0~35 d为愈伤组织形成期;35~65 d为不定根形成期;65~95 d为不定根伸长期。50 a侧柏以南面上部的插穗生根效果较好,生根率为40.8%。100 a侧柏6月扦插的生根率为20.1%。5 a用质量浓度600 mg/L的ABT1处理4 h时的生根效果最好,而100、300、700 a侧柏插穗用中浓度的IBA+NAA (1:1)处理时扦插生根率较高。进一步筛选发现质量浓度为1 000 mg/L的IBA+NAA (等质量比)处理1 min的插穗生根效果最好。全部年龄段侧柏插穗在愈伤组织形成期,可溶性糖、淀粉含量都上升,而5 a侧柏插穗上升的幅度最大。【结论】 选择侧柏古树幼嫩枝条夏季扦插有利于促进插穗不定根的形成。

Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material

【Objective】Improve the rooting rate and establish the wig cutting propagation of ancient Platycladus orientalis.【Method】In this study,a total of 3 treatment groups using three different concentrations(200-600 mg/L,1000-3000 mg/L and 4000-10000 mg/L)of ABT1,NAA or IBA+NAA(mass ratio 1:1)were used to treat 5-,100-,300-,700-year-old Platycladus orientalis cuttings.The rooting process,rooting(rooting rate,rooting number,the longest root length)and the dynamic changes in nutrient content(soluble sugar,soluble starch)were observed during the rooting process of Platycladus orientalis cuttings.【Result】The observations revealed that 0-35 days was the formation stage,the 35-65 days was adventitious roots formation stage,and 65-95 days was adventitious roots elongation stage.The southern upper cuttings from the 50-year-old P.orientalis exhibited the best rooting percentage of 40.8%.The rooting rate of the 100-year-old P.orientalis was the highest in June with 20.1%.Low concentration growth regulator treatment of ABT1(600 mg/L)for 4 h was beneficial to root formation of cuttings from 5-year-old P.orientalis,and a mixture solution of medium concentration IBA+NAA(moss ratio 1:1)treatment is good for rooting of cuttings from 100-,300-and 700-year-old P.orientalis.Further studies revealed that the optimal mixture solution of IBA+NAA(moss ratio 1:1)of 1000 mg/L for 1 min could improve the rooting rate of cuttings from the ancient P.orientalis.For all age groups,the content of soluble sugar and starch increased during callus formation stage;the 5-year-old P.orientalis showed the maximum increase.【Conclusion】Nutrient content of the branches are relatively higher in summer,which is beneficial for increasing the rooting rate of P.orientalis cuttings.