不同生长调节物质对宣木瓜愈伤组织的诱导

以宣木瓜(Chaenomeles speciosa Nakai)种子的子叶、胚轴为外植体,研究不同生长调节物质对愈伤组织诱导的影响.结果表明:(1)在子叶诱导实验中,2,4-D比NAA更高效地诱导出愈伤组织,且愈伤组织生长量大,经过继代培养后仍能保持较好的长势.2,4-D与KT组合的效果好干2,4-D与BA的组合.筛选试验表明:MS 2,4-D(1.0 mg/L) KT(0.1 mg/L)对子叶愈伤组织诱导及其生长效果较好.(2)胚轴愈伤组织诱导率达到87%,而子叶的诱导率较低,为69%.虽然NAA的诱导效果较好,但愈伤组织生长速度较慢;2,4-D能高效诱导愈伤组织,但容易出现玻璃化.提高NAA,2,4-D和BA的浓度,愈伤组织呈现水浸状的比例和程度都会相应提高.试验中,2,4-D(0.1 ng/L) BA(0.3 mg/L)处理对胚轴愈伤组织的诱导效果较好.

Effect of Different Growth Regulators on Callus Induction of Chaenomeles speciosa Nakai

The effects of different types and concentrations of plant growth regulators on callus tissue induction of cotyledons and hypocotyls of Chaenomeles speciosa Nakai were studied. The results showed that 2,4- D had a better effect on inducing and subculture callus tissue than that of NAA in the experiment of cotyledons. A satisfactory result of callus tissue induction and callus growth was achieved on MS medium supplemented with 1.0 mg/L 2,4-D and 0. 1 mg/L KT. Callus tissue induction rate of hypocotyls was 87G, which was higher than that of cotyledons, i. e. , 69%. The proportion and the degree of callus tissue vitrifaction would scale up when the concentration of auxin and BA increased. Best callus tissue of hypocotyls were obtained on MS medium contained 0.1 mg/L 2,4-D and 0.3 mg/L BA.