| 刺槐根瘤菌侵入线研究中的电镜制样方法 |
| |
| 引用本文： | 甘习华,韩素芬.刺槐根瘤菌侵入线研究中的电镜制样方法[J].南京林业大学学报(自然科学版),1997,21(4):90-92. |
| |
| 作者姓名： | 甘习华 韩素芬 |
| |
| 作者单位： | 南京林业大学电镜室 |
| |
| 摘 要： | 介绍一显示刺槐根瘤中侵入线超微结构的电镜制方法。以透射电镜制样方法为基础，将制好的刺槐根瘤树脂块，在同一部位用超薄切片机制成厚切片和超薄切片。厚切片经脱树脂后，扫描电镜观察；超薄切片经染色后，透射电镜镜检。
|
| 关 键 词： | 透射电镜 扫描电镜 刺槐 侵入线 根瘤菌 |
SPECIMEN PREPARATION FOR ELECTRON MICROSCOPY IN THE STUDY ON THE INFECTION THREADS OF THE HOST CELL OF ROBINIA PSEUDOACACIA |
| |
| Gan Xihua,Han Sufen.SPECIMEN PREPARATION FOR ELECTRON MICROSCOPY IN THE STUDY ON THE INFECTION THREADS OF THE HOST CELL OF ROBINIA PSEUDOACACIA[J].Journal of Nanjing Forestry University(Natural Sciences ),1997,21(4):90-92. |
| |
| Authors: | Gan Xihua Han Sufen |
| |
| Institution: | Election Microscopy Laboratory Nanjing Forestry University Nanjing 210037 |
| |
| Abstract: | This paper introduced a method of specimen preparation for electron microscopy which showed the ultrastructure of the infection threads in the host cell of Robinia pseudoacacia . Based on the specimen preparation of TEM, thick sections and ultrathin sections were made at the same location on nodule embeded by Epon_812. Thick sections were de_resined and coated with gold_palladium for SEM; ultrathin sections were stained for TEM. The result showed specimens prepared by this method could obtain ultrastructrue image and three_dimensional image at the same location while infection threads in the host cell of Robinia pseudoacacia . |
| |
| Keywords: | TEM SEM Robinia pseudoacacia Infection thread |
| 本文献已被 CNKI 维普 等数据库收录！ |
