黄河三角洲柽柳群体遗传多样性RAPD分析

采用随机扩增多态DNA(RAPD)分子标记技术,分析了黄河三角洲主要优势树种之一柽柳(Tamarix chinensis)3个天然群体的遗传多样性及遗传分化。结果表明:26条随机引物扩增出105个可分析位点,多态位点百分比40.07%,Nei的基因多样度(h)为0.4061,Shannon多样性指数(I)为0.5917,基因分化系数(Gst)为0.0507,基因流值为9.3564。分子方差分析(AMOVA)表明,在总遗传变异中,群体间遗传变异占7.17%,群体内占92.83%。说明柽柳物种内存在较高的遗传多样性,群体的遗传变异主要存在于群体内,群体间基因交流频繁,遗传距离与地理距离具有显著相关性。

RAPD analysis of population genetic diversity of Tamarix chinensis in Yellow River delta

In this paper genetic diversity and differentiation of 3 natural populations of Tamarix chinensis,one of the main predominated tree species in Yellow River Delta,were studied using random amplified polymorphic DNA(RAPD) techniques.It was showed that amplifications with 26 RAPD primers yielded 105 analyzable loci and the proportion of polymorphic loci accounted for 40.07%.Nei's gene diversity(h),Shannon diversity index(I),Coefficient of genetic differentiation(Gst) and gene flow were 0.406 1,0.591 7,0.050 7 and 9.356 4,respectively.AMOVA showed that there was a relatively low level(7.17%) genetic differentiation among the populations.Therefore,a relatively high level of genetic diversity existed in species.The majority of genetic variation occurred in populations.A high level of gene flow happened among populations.Genetic distance was remarkably correlated with geographic distance.