柳蓝叶甲生物碱感器的定位及电镜观察

为测定柳蓝叶甲感知生物碱感器的部位,建立了相应的生测方法。通过用化学方法使不同器官上的感器失活后,了解生测处理的试虫在生物碱处理的杨树叶上和对照叶上的产卵偏好,确定其感知生物碱的感器部位。生测结果表明:柳蓝叶甲感知生物碱的化学感器主要位于柳蓝叶甲成虫下颚须、下唇须和前足跗节上,而触角上感器对产卵选择无任何影响。在生测基础上对下颚须、下唇须和前足跗节上的感器进行扫描电镜观察。结果发现,在柳蓝叶甲下颚须、下唇须和前足跗节上都只有单一感器存在。可推断柳蓝叶甲感知生物碱感器可能就是下颚须、下唇须顶端的栓锥感器和足跗节顶端的毛状感器。

Location and SEM Observation of Sensilla for Quinolizidine Alkaloids in Plagiodera versicolora

To locate the organs on which the quinolizidine alkaloid sensilla of Plagiodera versicolora are distributed,bioassay methods were set up.Cutting off the organs physically or denaturing of the sensilla chemically on the organs,then bioassaying oviposition selection on alkaloid treated and untreated leaves in treated adults to determine the organs on which the alkaloid sensilla were located.The results of the bioassay by chemically denaturing the sensilla of the possible organs showed that the sensilla for the alkaloid in P.versicolora were mainly located on maxillary palpi,labial palpi,and tarsi of the fore legs.However,denaturing of the sensilla on the antenna had no effects on the oviposition selection of the adults.Based on the results of the bioassay,the sensilla on mandibles,maxillae and tarsi of the legs were observed by Scanning Electron Microscope(SEM).The results showed that there was only single type of sensilla on the tips of maxillary palpi,labial palpi.Therefore,the alkaloid sensilla of P.versicolora were likely the styloconic sensillum on the tips of maxillary palpi,labial palpi and the trichoid sensilla on the tarsi of the fore legs.