| 离体培养西葫芦子叶花芽分化最佳龄期的选择 |
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| 引用本文： | 黄作喜,沈惠娟,谢寅峰.离体培养西葫芦子叶花芽分化最佳龄期的选择[J].南京林业大学学报(自然科学版),2001,25(6):15-18. |
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| 作者姓名： | 黄作喜 沈惠娟 谢寅峰 |
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| 作者单位： | 1. 云南省农科院园艺所,云南昆明,650205
2. 南京林业大学,江苏南京,210037 |
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| 基金项目： | 国家自然科学基金资助项目（39770604） |
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| 摘 要： | 为研究花芽分化的机理，选择西葫芦种苗在一定真叶期时的子叶为外植体，培养于附加KT1.0mg/L MS培养基中，其花芽分化率可达60%；分析葫芦种苗在一片真叶期时子叶内源激素和多胺含量，发现子叶内源GA1/3含量偏低，而ZRs含量剧增，同时ZRs/GA1/3、ABA/GA1/3、Spd/Put、Spm/Put比值较高，这些内源因素有利于西葫芦子叶花芽分化。
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| 关 键 词： | 花芽分化 西葫芦 子叶 离体培养 内源激素 多胺含量 龄期 成花机理 |
| 文章编号： | 1000-2006(2001)06-0015-04 |
| 修稿时间： | 2001年4月30日 |
Studies on the Floral Bud Differentiation of Cotyledon of Cucurbita Pepo L. in Vitro |
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| HUANG Zuo-xi,SHEN Hui-juan,XIE Yin-feng.Studies on the Floral Bud Differentiation of Cotyledon of Cucurbita Pepo L. in Vitro[J].Journal of Nanjing Forestry University(Natural Sciences ),2001,25(6):15-18. |
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| Authors: | HUANG Zuo-xi SHEN Hui-juan XIE Yin-feng |
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| Institution: | HUANG Zuo-xi1,SHEN Hui-juan2,XIE Yin-feng2 |
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| Abstract: | We chose the cotyledous of cucurbit seedlings during first real leaf emerged as explant. KT 1.0 mg/L as a ideal hormone treatment in which a floral experimental system of cucurbit cotyledon was established in vitro. This system showed good repeat and higher ratio of floral bud differentiation (60%). The content of endogenous GA1/3 in the cotyledons is lower during first real leaf of cucurbit seedlings, but ZRs increased significantly, meanwhile ZRs/GA1/3 ABA/GA1/3 Spd/Put Spm/Put showed higher ratio which benefit floral bud differenfiation of cotyledons. So that it is ideal seedlings age to cut cotyledon during first real leaf emerged of cucurbit seedlings. |
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| Keywords: | Floral bud differentiation Hormones Polyamines Cucurbita pepo L |
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