基于SSR标记的丛生竹杂种鉴定、遗传分析和指纹图谱构建

【目的】 准确鉴定丛生竹杂交种,分析杂种及其亲本的遗传关系,开发可以利用的指纹图谱。【方法】 以孝顺竹(Bambusa multiplex)×麻竹(Dendrocalamus latiflorus)、孝顺竹(B. multiplex)×粉单竹(B. chungii)两个杂交群体为材料,从已公布的竹子SSR引物中随机选取30对引物进行筛选、检测和分析。【结果】 发现6对SSR引物(P8、P9、P18、P19、P20、P27)适于孝顺竹×麻竹组合的杂种鉴定和遗传分析,鉴定结果表明34个子代全部为真实杂种,杂种真实率为100%;另外6对SSR引物(P2、P9、P10、P11、P20、P28)则适于孝顺竹×粉单竹组合的杂种鉴定和遗传分析,鉴定结果表明59个子代中有54个真实杂种,杂种真实率为91.5%。孝顺竹×麻竹的杂种遗传了较多的母本位点,而孝顺竹×粉单竹的杂种则遗传了较多的父本位点。利用SSR引物扩增出的位点分别构建了两个杂交群体的指纹图谱,为品种保护提供了保障。【结论】 SSR引物能有效地鉴定丛生竹杂交种的真伪,父母本的遗传位点在不同杂交组合的子代中遗传概率不同,杂交群体指纹图谱的构建可为品种保护提供保障。

SSR-based hybrid identification,genetic analyses and fingerprint development of hybridization progenies from sympodial bamboo (Bambusoideae,Poaceae)

【Objective】 The study aims to accurately verify sympodial bamboo hybrids, clarify genetic relationships between hybrids and their parents, and develop applicable fingerprints. 【Method】 We screened, tested and analyzed 30 pairs of randomly selected published bamboo simple sequence repeat (SSR) primers using hybrids derived from Bambusa multiplex × Dendrocalamus latiflorus and B. multiplex × B. chungii crosses. 【Result】 According to the results, six SSR primer pairs (P8, P9, P18, P19, P20 and P27) were suitable for the hybrid identification and genetic analysis of the B. multiplex × D. latiflorus cross, which revealed that all 34 progenies were true hybrids (100%). Six SSR primer pairs (P2, P9, P10, P11, P20 and P28) were suitable for the hybrid identification and genetic analysis of the B. multiplex × B. chungii cross, which revealed that 54 of the 59 progenies were true hybrids (91.5%). Hybrids of the B. multiplex × D. latiflorus cross inherited more maternal alleles, while hybrids of the B. multiplex × B. chungii cross inherited more paternal alleles. The alleles detected using SSR primers were used to construct hybrid fingerprints of the two crosses for variety protection. 【Conclusion】 The SSR primers could be used to effectively verify sympodial bamboo hybrids as the progeny inherited alleles from different parents at varying probabilities in different crosses. In addition, the development of hybrid fingerprints could facilitate variety protection.