薄壳山核桃嫁接愈合过程中差异蛋白质的分析

【目的】嫁接是繁殖薄壳山核桃的重要手段,拟从蛋白质水平揭示薄壳山核桃嫁接愈合的机理。【方法】利用双向电泳技术结合MALDI-TOF/TOF-MS,研究了薄壳山核桃嫁接愈合部位4个发育时期(嫁接后第1、6、10和25天)的差异蛋白。【结果】共成功鉴定48个差异蛋白,这些差异蛋白按功能可分为7类,包括能量代谢、抗性及防御、细胞生长、次生代谢、蛋白质合成、氨基酸代谢以及未知功能蛋白。【结论】果糖二磷酸醛缩酶、磷酸甘油酸激酶、丙酮酸脱羧酶、三磷酸腺苷酶能够为嫁接愈合提供所需的能量; 抗坏血酸过氧化物酶、过氧化物还原酶能够有效清除嫁接愈合过程中积累过多的活性氧; 可溶性无机焦磷酸酶可以促进愈伤组织的增殖; 类胱天冬蛋白酶、α微管蛋白可能参与管状分子的分化。

Analysis of the differential proteins in the graft healing process of pecan (Carya illinoinensis)

Objective]Grafting is an important method for propagating pecan. This paper aimed to reveal the mechanism underlying graft healing on the proteomic level. Method] We investigated the differential proteins in the graft union of pecans at four developmental stages (1, 6, 10 and 25 days after grafting) based on two-dimensional gel electrophoresis and MALDI-TOF/ TOF-MS. Result] In total, 48 differential proteins were identified, and these identified proteins were classified into seven functional categories: energy metabolism, stress and defense responses, cell growth, secondary me-tabolism, protein synthesis, amino acid metabolism and function unknown. Conclusion ] Fructose-bisphosphate aldolase, phosphoglycerate kinase, pyruvate decarboxylase and adenosine triphosphatase could provide adequate energy for the graft healing of pecan. Ascorbate peroxidase and peroxiredoxins can eliminate the excessive reactive oxygen spe-cies caused by grafting. Soluble inorganic pyrophosphatase may play a role in promoting callus proliferation. Metacaspase and alpha tubulin may be associated with tracheary element differentiation.