理化因素对风信子花色苷稳定性的影响

为探讨理化因子对风信子花色苷稳定性的影响,采用体外试验,以风信子‘Woodstock’花瓣为材料,研究了温度、光照、pH和金属离子等各因素对风信子花瓣中花色苷呈色及光谱特征变化规律的影响。结果表明:风信子‘Woodstock’花色苷不耐高温和强光,温度升高及延长加热时间均使花色苷降解褪色,且随着pH增大,花色苷最大吸收波长朝长波方向移动;Fe~(3+)、Fe~(2+)、Al~(3+)和Cu~(2+)均使花色苷变色,但Mg~(2+)对其无明显影响,Ca~(2+)和Zn~(2+)则在高浓度时有增色效应,Pb~(2+)会破坏花色苷的稳定性而产生白色沉淀。除了Fe~(2+)和Pb~(2+)产生蓝移外,其他金属离子都使花色苷发生不同程度的红移。

Effects of physical and chemical factors on anthocyanin stability in Hyacinthus

In order to investigate the effect of physical and chemical factors on anthocyanin stability in Hyacinthus, the petals of hyacinthus‘Woodstock’ was used to study the effects of temperature, light, pH and metal ions on the changing patterns of the color of anthocyanin extraction and spectral characteristics by experiments in vitro. The results showed that the anthocyanidin from hyacinthus petals was not tolerant to high temperature and strong light, and pH exerted remarka?ble effects on anthocyanins since there was a bathochromic shift of the maximwm absorption warelengte as the increase of pH value. Fe3+, Fe2+, Al3+ and Cu2+ changed the color of anthocyanins, but the influence of Mg2+ was not significant. High concentration of Ca2+and Zn2+produced the hyperchromicity effect, while Pb2+created white precipitation in antho?cyanin solution. High concentration of Fe2+and Pb2+caused the maximum wavelength blue shift, yet the other metal ions showed bathochromic shift effect with the increase concentration to various degrees. These results could provide some sci?entific basis to explore the mechanism of flower color formation in hyacinthus, identifying the structure of the hyacinthus anthocyanin and further utilizing the hyacinthus pigment.