| 杨树抗杨四瘿螨相关基因的表达分析 |
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| 引用本文： | 张萍,潘惠新,黄敏仁,王明庥.杨树抗杨四瘿螨相关基因的表达分析[J].南京林业大学学报(自然科学版),2009,0(4):8-12. |
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| 作者姓名： | 张萍 潘惠新 黄敏仁 王明庥 |
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| 作者单位： | 南京林业大学,江苏省杨树种质创新与品种改良重点实验室,江苏,南京,210037 |
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| 基金项目： | 国家"十一五"科技支撑计划，国家高技术研究发展计划 |
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| 摘 要： | 采用抑制性消减杂交(suppression subtractive hybridization，SSH)技术，在构建杨四瘿螨诱导差异表达的抑制消减cDNA文库的基础上，通过RTPCR技术对候选基因进行了验证，其中有6条差异表达候选基因在4个时间点表达量有明显的增强。运用RACE(Rapid Amplification of cDNA Ends)技术克隆获得了一段全长2 053 bp的cDNA克隆，命名为PtWRKY，编码588个氨基酸。
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| 关 键 词： | 杨四瘿螨 RTPCR RACE PtWRKY |
Differentially expressed gene analysis of populus induced by Tetra lobulifera(Keifer) |
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| ZHANG Ping,PAN Hui-xin,HUANG Min-ren,WANG Ming-xiu.Differentially expressed gene analysis of populus induced by Tetra lobulifera(Keifer)[J].Journal of Nanjing Forestry University(Natural Sciences ),2009,0(4):8-12. |
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| Authors: | ZHANG Ping PAN Hui-xin HUANG Min-ren WANG Ming-xiu |
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| Institution: | Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China |
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| Abstract: | A suppression subtractive hybridization(SSH) cDNA library was constructed with Populus deltoides induced by Tetra lobulifera(Keifer).Real-time PCR was performed to confirm the candidate genes.In this study,six differentially expressed genes were found to be up-regulated.Subsequently,the full-long cDNA sequence about 2 053 bp was cloned by the RACE(Rapid Amplification of cDNA Ends) and named PtWRKY,which encod 588 amino acids. |
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| Keywords: | RT-PCR RACE PtWRKY |
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