AFLP分子标记在鉴定海滨锦葵杂交后代上的应用

海滨锦葵是较好的食用和工业利用的盐生经济植物。用 AFLP分子标记对海滨锦葵两对杂交亲本的36个F1 代个体的真伪进行了鉴定。18个AFLP引物对在亲本Hy103×Hh182间扩增出1 100条AFLP带,其中108条是多态带(多态率为9.82%);在亲本Slhy353×Blho301间扩增出1 181个AFLP条带,其中148条是多态带(多态率为12.51%)。实验结果表明:(1)引物对E AAC / M CTC在亲本Hy103×Hh182间扩增出5条父本特征带:401 bp,390 bp,156 bp,137 bp和108 bp,引物对 E AAG/M CTG则产生了7条:457 bp,356 bp,312 bp,251 bp,240 bp,190 bp和76 bp。父本特征带从Hy103×Hh182的8个F1 代个体均扩增出,表明其为真杂种;(2)引物对E AAC/M CTT在亲本 Slhy353×Blho301 间扩增出 5 条父本特征带:440 bp,393 bp,289 bp,162 bp和67 bp,引物对 E ACA/M CAT 则产生了 4 条: 448 bp, 360 bp, 217 bp和 145 bp;父本特征带从Slhy353×Blho301 28个F1 代个体中的26个个体上扩增出,表明其为真杂种,另外2个为假杂种。

Identification of Hybrids of Kosteletzkya virginica Using AFLP Markers

Kosteletzkya virginica is a potential halophytic grain crop for consumption and industrial utilization. AFLP markers were used to identify 36 F1 hybrids of two parents. Eighteen peimer combinatios amplified 1100 AFLP band between parent Hy103and Hh182,108(9.82%)of which were polymorphic. Total 1182AFLPP bands were amplified between parent Slhy353 and Blho301 ,148(12.51%) of which were polymorphic. primer combination E+AAC/M+CTC amplified five paternal characteristic bands between Hy103 and Hh182:401bp,390bp,156bp,137bpand 108bp,whereas E+AAC/M+CTT generated seven paternal characteristic bands :457bp,356bp,312bp,251bp,190and 176bp. All these paternal characteristic bands were found in eight F1bhybrids of Hy103×Hh182 ,which were real hybrid .Primer combinatoin E+AAC/M+CTT amplified five paternal characteristic bands between Slhy353 and Blho301:440bp, 393bp,289bp,162bpand 67bp, whereas E+ACA/M+CAT kgenerated four characteristic bands :448bp,360bp,217bpand 145bp.All these paternal characteristic four characteristic bands:448bp,360bp,217bpand 145bp.All these paternal characteristic bands were found in 26of 28F1 hybrids of slhy353×Blho301,and other two F1 hybries were false hybrids.