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苯酚高效降解菌的筛选及其降解特性研究
引用本文:刘建忠,王乾,颜家保,秦晓蓉,李凌凌.苯酚高效降解菌的筛选及其降解特性研究[J].武汉科技大学学报(自然科学版),2011,34(4).
作者姓名:刘建忠  王乾  颜家保  秦晓蓉  李凌凌
作者单位:武汉科技大学煤转化与新型炭材料湖北省重点实验室,湖北武汉,430081
基金项目:湖北省科技厅重点研发项目,武汉市科技供需对接计划资助项目
摘    要:从某焦化厂处理废水的活性污泥中筛选到一株苯酚高效降解菌(Wust-C),该菌属革兰氏阴性菌.对Wust-C降解苯酚的特性进行试验研究.结果表明,在初始苯酚浓度为1 000 mg/L的试样中,培养24 h后,Wust-C对苯酚的降解率可达98%;初始苯酚浓度为300 mg/L试样中的苯酚可在8h内完全降解.采用海藻酸钠对菌体进行固定化后,Wust-C的降酚效率进一步得到提高.培养24 h后,固定化Wust-C对初始苯酚浓度为1 200 mg/L试样中的苯酚降解率达到100%,初始苯酚浓度为300 mg/L试样中的苯酚可在4h内完全降解.Wust-C的加入对混合菌群降解苯酚起到了促进作用.

关 键 词:苯酚  生物降解  固定化  混合菌群

Screening and degrading properties of a highly-efficient phenol-degradation bacterial strain
Liu Jianzhong,Wang Qian,Yan Jiabao,Qin Xiaorong,Li Lingling.Screening and degrading properties of a highly-efficient phenol-degradation bacterial strain[J].Journal of Wuhan University of Science and Technology(Natural Science Edition),2011,34(4).
Authors:Liu Jianzhong  Wang Qian  Yan Jiabao  Qin Xiaorong  Li Lingling
Institution:Liu Jianzhong,Wang Qian,Yan Jiabao,Qin Xiaorong,Li Lingling(Hubei Coal Conversion and New Carbon Materials Key Laboratory,Wuhan University ofScience and Technology,Wuhan 430081,China)
Abstract:A bacterial strain with high phenol degradation property was obtained from the activated sludge of a local coke plant.The strain was confirmed to be Gram-negative and named as Wust-C.Its phenoldegradationpropertywascharacterized.Theresultsshowthedegradatonrateofphenol(1 000 mg/L) in 24 h is as high as 98.0%.The phenol in basal media(300 mg/L) is thoroughly degraded within 8 h.After immobilization with sodium alginate,the degradation efficiency is further raised.The phenol(1 200 mg/L) in a basal media is com...
Keywords:phenol  biodegradation  immobilization  mixed bacterial consortia  
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