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| 抗白血病转移药物筛选模型的实验研究 | |
| 引用本文： | 王强,舒榕,刘红云,沈关心.抗白血病转移药物筛选模型的实验研究[J].武汉科技大学学报(自然科学版),2006,29(2):189-192. |
| 作者姓名： | 王强 舒榕 刘红云 沈关心 |
| 作者单位： | 1. 武汉科技大学医学院,湖北,武汉,430080 2. 湖北省中山医院,湖北,武汉,430030 3. 华中科技大学同济医学院,湖北,武汉,430030 |
| 摘 要： | 构建抗白血病转移药物筛选模型.K562细胞转染GFP质粒后,经筛选,将稳定高表达GFP的K562细胞注射到鸡胚里,孵化后观察K562细胞在鸡胚中转移情况.在其脑部、胸骨及心脏可看到明显的荧光,最频繁发生的部位是脑部.成功构建含GFP的白血病鸡胚模型,并验证了虎眼万年青皂甙（OSW-1）和链激酶（SK）联合用药的抗转移效果. |
| 关 键 词： | 鸡胚 绿色荧光蛋白 转移 筛选 |
| 文章编号： | 1672-3090(2006)02-0189-04 |
| 收稿时间： | 2005-09-02 |
| 修稿时间： | 2005年9月2日 |
Screening of Antimetastatic Agents for Leukaemia: An Experimental Research |
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| WANG Qiang,SHU Rong,LIU Hong-yun,SHEN Guan-xin.Screening of Antimetastatic Agents for Leukaemia: An Experimental Research[J].Journal of Wuhan University of Science and Technology(Natural Science Edition),2006,29(2):189-192. | |
| Authors: | WANG Qiang SHU Rong LIU Hong-yun SHEN Guan-xin |
| Institution: | 1. Medical College, Wuhan University of Science and Technology, Wuhan 430081, China; 2. Hubei Zhongshan Hospital, Wuhan 430030, China; 3. Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| Abstract: | An imageable fluorescent chick-embryo metastatic cancer model was established with the use of green fluorescent protein(GFP).K562 cells which were transfected with GFP-N2 and screened were injected in the chick embryo.GFP-K562 carcinoma metastases were visualized by fluorescence,and GFP-K562 was visualized in the brain and sternum of the developing chick embryo,with the most frequent site being the brain.The combination of streptokinase and OSW-1 has been evaluated in the GFP metastatic model. |
| Keywords: | K562 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |