聚乙烯醇静电纺丝法固定葡萄糖氧化酶

利用静电纺丝纳米纤维具有高比表面积和多孔疏松结构的优势固定葡萄糖氧化酶，以提高酶电极的性能．通过聚乙烯醇（PVA）和葡萄糖氧化酶（GOD）共同静电纺丝的方法在金电极表面获得了固定化酶膜，用于构筑安培型葡萄糖生物传感器，膜的红外光谱、紫外-可见光谱和扫描电镜的分析均表明酶成功固定在静电纺丝形成的纳米纤维膜中．循环伏安测试表明固定化酶在静电纺丝纳米纤维中保持了活性，采用PVA静电纺丝法固定COD比利用浇铸膜法所得到的酶修饰电极对葡萄糖有更好的电流响应特性，通过在静电纺丝溶液中加入纳米金进一步提高了酶电极的电化学响应特性．

Immobilization of Glucose Oxidase by Poly ( Vinyl Alcohol) Electrospinning

A novel technique for enzyme immobilization by electrospinning poly(vinyl alcohol)(PVA)and glucose oxidase(GOD)on the surface of the Au electrode for amperometric biosensor was presented.The elec- trospun nanofibers were used as enzyme immobilization material to improve the performance of the amperometrie enzyme electrode owing to high specific surface and porous structure.Infrared spectroscopy,ultraviolet-visible spectrum and scanning electronic microscope of the membranes showed that GOD had been immobilized inside the electrospun PVA membranes.Cyclic voltammetry measurement demonstrated that the immobilized GOD re- mained active inside the electrospun PVA fibrous membranes,and the electrochemistry response performance of the eleetrospun fibrous enzyme membrane was better than that of the casting enzyme membrane.Au nanoparti- cles were introduced inside the electrospun membranes as the electron medium to enhance the catalytic activity of the immobilized enzyme.