| HAK基因对玉米的遗传转化及其耐盐性研究 |
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| 引用本文： | 季静,汪婷婷,王罡,吴疆,关春峰.HAK基因对玉米的遗传转化及其耐盐性研究[J].天津大学学报(自然科学与工程技术版),2013(7):659-665. |
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| 作者姓名： | 季静 汪婷婷 王罡 吴疆 关春峰 |
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| 作者单位： | 天津大学化工学院;天津大学遗传工程研究所 |
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| 基金项目： | 国家转基因生命新品种培育重大专项资助项目(2009ZX08003-019B);国家转基因生物新品种培育重大专项资助项目(2011ZX08003-005);国家自然科学基金资助项目(31271793,31271419);教育部高等学校博士学科点专项科研基金资助项目(20100032110060) |
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| 摘 要： | 摘要：用农杆菌介导法将高亲和性钾离子转运体基因（HAK）和Bar基因转入5个优良玉米自交系7922、P138、265、238和271中，并对影响其遗传转化效率因素进行了优化．经PCR和RT-PCR检测证实获得阳性植株．除草剂涂抹实验证明Bar基因已经整合进玉米基因组．用不同浓度的盐溶液处理转基因植株和对照植株，发现转基因植株叶片中的K＋含量、脯氨酸和叶绿素含量均高于未转化植株，而Na＋含量低于未转化植株，表明HAK基因已经整合进玉米基因组，并通过过量表达提高了植株的耐盐性．
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| 关 键 词： | 玉米自交系 农杆菌介导 HAK 耐盐性 |
Genetic Transformation of Maize with HAK Gene and Its Effect on Salt Tolerance |
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| Ji Jing,Wang Tingting,Wang Gang,Wu Jiang,Guan Chunfeng.Genetic Transformation of Maize with HAK Gene and Its Effect on Salt Tolerance[J].Journal of Tianjin University(Science and Technology),2013(7):659-665. |
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| Authors: | Ji Jing Wang Tingting Wang Gang Wu Jiang Guan Chunfeng |
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| Institution: | 1.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;2.Research Institute of Genetic Engineering,Tianjin University,Tianjin 300072,China) |
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| Abstract: | The HAK and Bar genes were transferred into five different inbred lines of maize, including 7922, P138, 265, 238 and 271, by Agrobacterium-mediated transformation. Factors influencing transformation efficiency were optimized. Transgenic plants, which are resistant to phosphinothricin （PPT）, were acquired by PCR and RT-PCR. When treated with different concentration of NaC1, higer K＋, proline and chlorophyll contents were observed in transgenic leaves than in wild type leaves, but Na＋ content in transgenic leaves was obviously lower than in wild type leaves. These results show that tIAK gene is integrated into maize genome and transcribed normally. Over-expressing of HAK gene enhances the salt tolerance of transgenic maize. |
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| Keywords: | maize inbred line Agrobacterium-mediated HAK salt tolerance |
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