| 从rbcL基因序列研究明福1号及其近似种苏丹草的亲缘关系 |
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| 引用本文： | 黄可辉,郭琼霞,虞斌,吴珍泉.从rbcL基因序列研究明福1号及其近似种苏丹草的亲缘关系[J].武夷科学,2004,20(1):139-143. |
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| 作者姓名： | 黄可辉 郭琼霞 虞斌 吴珍泉 |
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| 作者单位： | 1. 福建出入境检验检疫局,福建,福州,350003
2. 福建农林大学,福建,福州,350002 |
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| 摘 要： | 本实验通过对明福 1号及其近似种苏丹草的叶绿体 rbc L基因序列的测定和变异位点的分析 ,结果表明 :明福 1号 rbc L序列的 G+ C百分含量为 4 2 .93% ,而苏丹草 rbc L序列的 G+ C百分含量为 4 3.0 9% ;明福1号与苏丹草的 rbc L 序列的变异位点很少 ,仅为 4个 ,分别位于第 12 4 bp、6 41bp、84 9bp、880 bp位点上 ,总变异位点占总性状位点 (96 3bp)的 0 .4 1% ;同时 ,明福 1号与苏丹草的相似系数为 0 .997,表明明福 1号与苏丹草有极高的亲缘关系。
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| 关 键 词： | 明福1号 苏丹草 rbcL |
| 文章编号： | 1001-4276-(2004)01-0139-05 |
| 修稿时间： | 2004年11月14 |
THE PHYLOGENETIC RELATIONSHIPS OF SORGHUM PROPINGUUM (Mingfu No.1) AND S.SUDANENSE:EVIDENCE FROM rbcL SEQUENCE ANALYSIS |
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| HUANG Ke-hui,GUO Qiong-xia,YU Yun,WU Zhen-quan.THE PHYLOGENETIC RELATIONSHIPS OF SORGHUM PROPINGUUM (Mingfu No.1) AND S.SUDANENSE:EVIDENCE FROM rbcL SEQUENCE ANALYSIS[J].Wuyi Science Journal,2004,20(1):139-143. |
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| Authors: | HUANG Ke-hui GUO Qiong-xia YU Yun WU Zhen-quan |
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| Institution: | Fujian Entry-Exit Inspection and Qurantine Bureau 350001 |
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| Abstract: | Based on rbcL gene sequences in nuclear ribosomal DNA and their nucleotide variations, analyses of Sorghum propinguum (Mingfu No.1) and S. sudanense were presented. Results showed that the contents of G+C based on rbcL from S.propinguum (Mingfu No.1) was 42.93% and S. sudanense was 43.09%. Alignment of the rbcL gene sequences were analyzed by DNAMAN 5.0, only 4 nucleotide variations were found in 124bp, 641bp, 849bp and 880bp. The number of variations was 0.41% of all positions. The Jaccard coefficient between S.propinguum (Mingfu No.1) and S. sudanense was 0.997. It suggested that S.propinguum (Mingfu No.1) was closely related to S. sudanense. |
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| Keywords: | Sorghum propinguum (Mingfu No 1) Sorghum sudanense rbcL |
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