| 小麦矮腥黑粉菌PCR快速检疫鉴定方法 |
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| 引用本文： | 王念武,周卫川,郭琼霞.小麦矮腥黑粉菌PCR快速检疫鉴定方法[J].武夷科学,2008(0). |
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| 作者姓名： | 王念武 周卫川 郭琼霞 |
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| 作者单位： | 福建出入境检验检疫局技术中心; |
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| 基金项目： | 福建科技重大专项专题(2006NZ0002-1);;福建科技厅自然科学基金项目(X0650003) |
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| 摘 要： | 设计一对特异性引物,对小麦矮腥黑粉菌(TCK)及其近似种进行PCR扩增,结果表明TCK可以扩增出200bp清晰条带,而其近似种未出现条带,该方法可以作为TCK的PCR快速检疫鉴定方法。
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| 关 键 词： | 小麦矮腥黑粉菌 PCR 小麦印度腥黑穗病菌 禾草腥黑粉菌 小麦网腥黑粉病菌 |
RAPID METHOD OF PCR FOR DWARF BUNT IDENTIFICATION |
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| WANG Nian-wu,ZHOU Wei-chuan,GUO Qiong-xia.RAPID METHOD OF PCR FOR DWARF BUNT IDENTIFICATION[J].Wuyi Science Journal,2008(0). |
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| Authors: | WANG Nian-wu ZHOU Wei-chuan GUO Qiong-xia |
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| Institution: | Fujian Entry-Exit Inspection and Quarantine Bureau;Fuzhou;350002 China |
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| Abstract: | Dwarf bunt(Tilletia Controversa Kuhn,TCK) is one of the most dangerous quarantine wheat diseases in the world and hardest to control.In this paper,a pair of specific primers were designed and the PCR products showed that only the two TCK samples amplified the 200bp specific band,the others were not any amplified quick identification of TCK. |
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| Keywords: | dwarf bunt Tilletia Controversa Kuhn TCK TCT TFL identification PCR |
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