美洲鲎miRNA的生物信息学挖掘与分析

利用miRBase数据库中已有动物的小RNA（miRNA），通过生物信息学方法对美洲鲎(Limulus polyphemus)表达序列标签（Expressed Sequences Tag,EST）和cDNA进行序列比对，挖掘美洲鲎miRNA，得到了 72个miRNA前体(pre-miRNA)、49个可编码成熟miRNA，隶属于40个miRNA家族。miRNA家族归类结果表明，无论在低等脊椎动物还是高等动物中，miRNA家族的存在都是相当保守的。以miR-10基因前体为例，分析其序列与其他物种同源序列的差异，显示miRNA序列的高度保守性。miR-10前体序列分析系统进化发现，物种聚类与传统分类亲缘关系一致。

Bioinformatic Identification of Conserved miRNA in Limulus polyphemus

In this study，bioinformatics method were used to identify microRNAs of Limulus polyphemus.The ESTs and cDNA sequences of L.polyphemus were aligned to the known animal miRNAs from miRBase.72 miRNA precursors (pre-miRNAs) and 49 mature miRNAs belonging to 40 miRNA families were identified.The classified analysis of miRNA family showed that mature miRNAs and precursor sequences are highly conserved，not only existing in the low class of invertebrates but also in high vertebrates.Analysis of sequences homology with other species based on miR-10 precursors indicated that the sequences of miRNA were fairly conserved.The phylogenetic analysis of miR-10 precursor sequences revealed that clustering mode was consistent with the traditional classification of related species.