| 天门冬多糖的分离纯化及其部分理化性质 |
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| 引用本文： | 张部昌,吴东儒.天门冬多糖的分离纯化及其部分理化性质[J].安徽大学学报(自然科学版),1994,18(2):88-93. |
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| 作者姓名： | 张部昌 吴东儒 |
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| 作者单位： | 安徽大学生物系 |
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| 摘 要： | 中药天门冬冷水提取物，通过脱蛋白、丙酮分级及柱层析纯化，得到三种级份多糖：ＰＡ－Ｗ、ＰＡ－Ｉ和ＰＡ－Ⅱ，凝胶柱层析测定，它们的分子量分别是１５８万、２１９万和２１９万。三种多糖中均含有葡萄糖，甘露糖和半乳糖，但各自的摩尔比相差很大。此外，ＰＡ－Ⅱ中还含有一种未知的糖醛酸。高碘酸氧化、Ｓｍｉｔｈ降解、红外光谱和１３Ｃ核磁共振谱表明，ＰＡ－Ⅱ含α和β两种构型，具有１→４和１→６两种糖苷键，二者比例为５～６：１，是一种具有高度分枝结构的半乳甘露聚糖。
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| 关 键 词： | 天门冬，多糖 |
Isolation,Purification and Some Properties of Polysaccharides from Asparagus Cochinchinensis |
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| Zhang Buchang;Wu Dongru;Tao Leping.Isolation,Purification and Some Properties of Polysaccharides from Asparagus Cochinchinensis[J].Journal of Anhui University(Natural Sciences),1994,18(2):88-93. |
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| Authors: | Zhang Buchang;Wu Dongru;Tao Leping |
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| Institution: | Department of Biology Anhui University |
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| Abstract: | The rough polysaccharide was extracted with cold water from tradition Chinese medicineAsparagus Cochinchinensis,and three polysaccharides,PA-W, PA-I and PA-Ⅱ with Mws 1.58×106, 2. 19×106and 2. 19×106respectively,were isolated by deproteination, acetone fractionation and column chromatography. They all contain glucose, mannose and galactosee, but the rstios are different. In addition, PA-Ⅱcontains a kind of unidentified glycouronic acid,Periodate oxidation, Smith degradation and spectra of IR and 13C NMR reveal PA-Ⅱ owns αand β configurations, and has 1→4 and 1→6 linkages in the ratio of 5～6: 1. |
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| Keywords: | Asparagus cochinchinensis polysaccharide |
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