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二甲亚砜(DMSO)对桃树叶肉细胞抗冻性的影响
引用本文:葛继志,张晋,黄海波,谢惠安.二甲亚砜(DMSO)对桃树叶肉细胞抗冻性的影响[J].安徽大学学报(自然科学版),1999,23(2):99-102.
作者姓名:葛继志  张晋  黄海波  谢惠安
作者单位:安徽大学生命科学学院,合肥,230039
摘    要:在冷冻条件下,利用7230分光光度计、显微摄影及IEF-PAGE等方法测定不同浓度的DMSO对桃树叶肉细胞的抗冻作用。发现(1)材料冷冻后再加入DMSO对细胞的抗冻作用不明显;(2)先用DMSO处理后对细胞有明显的抗冻作用,其中VDMSO∶VH2O=2∶3及VDMSO∶VH2O=1∶1的配比对桃树叶肉细胞有较好的效果。

关 键 词:二甲亚砜,桃树,叶肉细胞,抗冻性

The Research on the Frost- proof Characteristics of Dimethylsufonide to the Mesophyill Cells Pyrnus Chinsis
e Jizhi,Zhang jin,Hua Haibo.The Research on the Frost- proof Characteristics of Dimethylsufonide to the Mesophyill Cells Pyrnus Chinsis[J].Journal of Anhui University(Natural Sciences),1999,23(2):99-102.
Authors:e Jizhi  Zhang jin  Hua Haibo
Institution:Department of Biology Anhui UniversityHefei230039
Abstract:This experiment utilize such guotes as 7230-Visible spectophometer microphotogrophy.IEF-PAGE etc.,and dralis support from chart,data and photoes. It probes into the frost-poof action of DMSO to the mesophyll cells and we found:(1) The DMSO has no evident action to the cell after been frosted.(2) after handled by the DMSO. The plant cell has clear frost-proof action especially the F group which is the best in all the fest group. (3) The DMSO clots harm for the cell if the concertration as very high such as G group.
Keywords:dimefhyl  sulfoxic  mesophyll cess  frost-proof characteristics  
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