种质资源酯酶同工酶的研究

研究烟草种子酯酶同工酶酶谱，品种包括国外引进烤烟品种，国内选育的烤烟品种和安徽省地方晒烟品种。研究结果表明：烟草品种种子中酯酶具有遗传稳定的特性，其同工酶谱带丰富（一般７－１０条），品种间既在主要谱带上具有共同的模式，又在部分谱带上存在差异，个别品种带变异较大。在烟草种子酯酶同工酶谱中，Ａ系谱带出现频率高（大于８５％，其中Ａ５达到１００％），Ｂ系频率低（最低的Ｂ２只有４１％）。

Investigation of Esterase Isozymogram in Germplasm Resouces

Electrophoretic analysis of esterase isozyme in tobacco seeds from main varieties cultivated in china, such as G-28, NC89, k326, Honghuadajingyan, Anxun 44 etc were made. The results were shown as follows:The band type of esterase isozymogram in tobacco seeds was very genetically stable. Many band zones, generally 7-10 band zone,could be observed among tobacco varieties. There was a common band pattern of esterase isozymogram in tobacco varieties, while a great variation in band types was observed only in few varieties. Type A series of esterase isozyme band zones in tobacco seeds were the most common ones, representing more than 85% of the total accessiens analysed (A1=100%), while the frequency of type B series was was lower than 85% (the lowest B2 =41%)