| 抗猪脂肪细胞膜蛋白抗体的制备及测定 |
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| 引用本文： | 杨东,胡晓琼,李斌,曹慕岚,马丹炜.抗猪脂肪细胞膜蛋白抗体的制备及测定[J].安徽大学学报(自然科学版),2007,31(4):85-89. |
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| 作者姓名： | 杨东 胡晓琼 李斌 曹慕岚 马丹炜 |
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| 作者单位： | 1. 四川师范大学,生命科学学院,四川,成都,610066;2. 四川省畜牧科学研究院,四川,成都,610066 |
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| 摘 要： | 猪屠宰后,立刻取其背部皮下脂肪组织在37℃膜萃取液中匀浆,用差速离心法和密度梯度离心法提取脂肪细胞膜蛋白,经SDS-PAGE分析,背部脂肪细胞膜蛋白与其他组织细胞膜蛋白具有较大差异,但也有一些相同的膜蛋白.以猪背部脂肪细胞膜蛋白为抗原,免疫雄性家兔,制备抗猪脂肪细胞膜蛋白血清,经酶联免疫反应(ELISA)测定,血清效价达到1:12800.同样,用ELISA和Western-blotting测定抗体与其他组织细胞膜的交叉反应,结果显示:抗猪脂肪细胞膜抗体与其他组织细胞膜有交叉反应,但反应性不高.
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| 关 键 词： | 猪脂肪细胞 膜蛋白 抗体 |
| 文章编号： | 1000-2162（2007）04-0085-05 |
| 修稿时间： | 2006-12-27 |
Preparation and identification of antibodies against porcine adipocyte plasma membrane protein |
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| YANG Dong,HU Xiao-qiong,LI Bin,CAO Mu-lan,MA Dan-wei.Preparation and identification of antibodies against porcine adipocyte plasma membrane protein[J].Journal of Anhui University(Natural Sciences),2007,31(4):85-89. |
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| Authors: | YANG Dong HU Xiao-qiong LI Bin CAO Mu-lan MA Dan-wei |
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| Institution: | 1. School of Life Science,Sichuan Normal University,Chengdu 610066,China; 2. Sichuan Animal Science Academy,Chengdu 610066,China |
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| Abstract: | The adipose tissue extracted from crossbred pigs was homogenized in 37℃ membrane extractant.Membrane proteins of pig subcutaneous adipocyte and other tissue cells were extracted by differential centrifugation method and density gradient centrifugation method.Comparative analyses of membrane protein profiles between adipocyte and other tissue cell proteins was conducted by SDS-PAGE.Though componets of membrane proteins were different among pig subcutaneous and other tissue cells,some membrane proteins were identical.The antibodies against porcine adipocyte plasma membrane protein were produced by immunising with subcutaneous adipocyte plasma membrane protein as antigen,and its titer reached 1∶12800 detected by ELISA.Likewise low cross-reactivity of the antisera to other pig tissues could be detected by ELISA and Western-boltting. |
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| Keywords: | porcine adipocyte plasma membrane protein antibody |
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