| 天门冬糖蛋白的分离纯化及其糖肽键的研究 |
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| 引用本文： | 张部昌,赵帜平,袁华玲,陈祖智,毕建军.天门冬糖蛋白的分离纯化及其糖肽键的研究[J].安徽大学学报(自然科学版),1996(4). |
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| 作者姓名： | 张部昌 赵帜平 袁华玲 陈祖智 毕建军 |
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| 作者单位： | 安徽大学生物系 |
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| 摘 要： | 冷水浸提中药天门冬，提取物经丙酮分级，得到ＡＧ－ａ，ＡＧ－ｂ和ＡＧ－ｃ三个级份。ＡＧ－ｃ用ＤＥＡＥ—纤维素柱纯化，得到水洗脱级份ＡＧ－ｗ和０．５ＮＮａＣｌ洗脱级份ＡＧ－ｃ。凝胶柱层析和醋酸纤维薄膜电泳，均表明ＡＧ－ｃ为均一性糖蛋白。红外光谱显示，所有的天门冬糖蛋白级份均含有乙酰基团。β—消去反应检测，ＡＧ－ｃ中糖和蛋白质间的连接键为Ｏ—型糖肽键
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| 关 键 词： | 天门冬，糖蛋白，糖肽键 |
Study on Isolation, Purification and Glucopeptide Bond of Glucoprotein form Asparagus Cochinchinensis |
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| Zhang,Buchang,Zhao,Zhiping,Yuan,Hualing,Chen,Zuzhi,Bi,Jianjun.Study on Isolation, Purification and Glucopeptide Bond of Glucoprotein form Asparagus Cochinchinensis[J].Journal of Anhui University(Natural Sciences),1996(4). |
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| Authors: | Zhang Buchang Zhao Zhiping Yuan Hualing Chen Zuzhi Bi Jianjun |
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| Institution: | Department of Biology Anhui University Hefei 230039 |
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| Abstract: | The crude glucoprotein, named AG,was extracted with cold water from traditional chinese medicine Asparagus Cochinchinensis,and with acetone precipitation, three fractions, AG-a,AG-b and AG-c,were isolated. Applied to a column of DEAE-Cellulose, AG was devided into AG-w with water elution and AG-c with 0.5N sodium chloride elution. AG-c was homogeneous on gel chromatography and on acetyl fiber film electrophoresis. IR spectrums showed all plucoprotein fractions had O-acetyl groups on their polysaccharide parts. β- Elimination revealed the bond between the polysaccharide and the protein of AG-c was O-type glucopeptide one. |
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| Keywords: | Asparagus Cochinchinensis glucoprotein glucopeptide bond |
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