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| 基于ITS、atpB-rbcL序列的醉翁榆(Ulmus gaussenii)与相近榆属植物的亲缘关系 | |
| 引用本文： | 韩雪,智颖飙,张保卫,周忠泽,何家庆,王再岚,李红丽,李静敏.基于ITS、atpB-rbcL序列的醉翁榆(Ulmus gaussenii)与相近榆属植物的亲缘关系[J].安徽大学学报(自然科学版),2011,35(6):98-107. |
| 作者姓名： | 韩雪 智颖飙 张保卫 周忠泽 何家庆 王再岚 李红丽 李静敏 |
| 作者单位： | 1. 安徽大学生命科学学院,安徽合肥,230039 2. 中国人民大学环境学院,北京,100872 3. 北京林业大学自然保护区学院,北京,100083 |
| 基金项目： | 中国博士后基金资助项目(20060400551); 农业部草地开放实验室基金资助项目; 安徽大学人才引进基金资助项目(06124,204033) |
| 摘 要： | 濒危植物保护生物学研究已成为生态学领域的研究热点之一.为了廓清我国特有濒危植物醉翁榆(Ulmus gaussenii)与相近榆属植物的系统地位,用分子标记ITS和atpB-rbcL手段分析醉翁榆与其他榆属相关种的亲缘关系,结果表明:24种榆属植物atpB-rbcL扩增片段长度为735～834 bp,G+C的含量在29.3%～31.6%之间,并没有发生很大的偏差;基于ITS(包括5.8 s)扩增片段,醉翁榆与黑榆系的毛果旱榆、栓枝春榆以及榆系中的大果榆、脱皮榆(U.lamellosa)相近;基于atpB-rbcL扩增片段,醉翁榆与榆系中的大果榆、脱皮榆以及明陵榆的关系比较近,两种标记都表现出醉翁榆与黑榆系中变种毛果旱榆、栓枝春榆的关系比较近;atpB-rbcL的NJ和Bayesian树完全支持榆属组下的分类以及榆属系统的演化关系. |
| 关 键 词： | 醉翁榆 遗传随机性 atpB-rbcL ITS 系统树 |
The phylogenetic relationships between Ulmus gaussenii Cheng and its close relatives in genus Ulmus based on the ITS and atpB-rbcL |
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| HAN Xue,ZHI Ying-biao,ZHANG Bao-wei,ZHOU Zhong-ze,HE Jia-qing,WANG Zai-lan,LI Hong-li,LI Jing-min.The phylogenetic relationships between Ulmus gaussenii Cheng and its close relatives in genus Ulmus based on the ITS and atpB-rbcL[J].Journal of Anhui University(Natural Sciences),2011,35(6):98-107. | |
| Authors: | HAN Xue ZHI Ying-biao ZHANG Bao-wei ZHOU Zhong-ze HE Jia-qing WANG Zai-lan LI Hong-li LI Jing-min |
| Institution: | HAN Xue1,ZHI Ying-biao1*,ZHANG Bao-wei1,ZHOU Zhong-ze1,HE Jia-qing1,WANG Zai-lan2,LI Hong-li3,LI Jing-min1 (1.School of Life Science,Anhui University,Hefei 230039,China,2.School of Environment and Natural Resources,Renmin University of China,Beijing 100872,3.College of Nature Conservation,Beijing Forestry University,Beijing 100083,China) |
| Abstract: | Conservation biology research of endanged plant has become popular in ecology area.To explore the systematic position of Ulmus gaussenii Cheng in genus Ulmus,the authors analyzed the phylogenetic relationship of U.gaussenii and its related species in Ulmus based on DNA sequences of the chloroplase atpB-rbcL gene and the ITS region of nuclear ribosomal DNA.The sequence of atpB-rbcL in 24 species ranged from 735 to 834 and the total G+C content varied from 29.3% to 31.6%.The phylogenetic tree based on the atp... |
| Keywords: | Ulmus gaussenii Cheng genetic randomicity atpB-rbcL ITS phylogenetic tree |
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