| OPA柱前衍生法用于反相高效液相色谱分析氨基酸 |
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| 引用本文： | 邬安珍,孙自勇.OPA柱前衍生法用于反相高效液相色谱分析氨基酸[J].安徽大学学报(自然科学版),1991,15(3):64-68. |
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| 作者姓名： | 邬安珍 孙自勇 |
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| 作者单位： | 南京师范大学理化中心，南京大学生化系 |
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| 摘 要： | 本文采用邻苯二甲醛(OPA)柱前衍生的反相高效液相色谱法,以甲醇、0.1M柠檬酸缓冲液梯度洗脱,分析了细胞色素C酶解产物的氨基酸组成。结合原子吸收光谱,确证了铂在细胞色素C上的修饰作用位点。
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| 关 键 词： | 邻苯二甲醛 柱前衍生 反相高效液相色谱 修饰细胞色素C 荧光标记 |
Analysis of Amino Acids with Precolumn Derivatization of O-phthaldialdehyde and Reversed-phase High Performance Liquid Chromatography |
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| Wu Anzheng Sun Ziyong.Analysis of Amino Acids with Precolumn Derivatization of O-phthaldialdehyde and Reversed-phase High Performance Liquid Chromatography[J].Journal of Anhui University(Natural Sciences),1991,15(3):64-68. |
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| Authors: | Wu Anzheng Sun Ziyong |
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| Institution: | Wu Anzheng Sun Ziyong |
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| Abstract: | The amino acids composition of peptides isolated from tryptic hydrolysate of cytochrome c modified by pt has been analysed by RP—HPLC with a methanoI gradient in O.1M aqueous sodium citrate.With the analysis of atomic absorption spectrum, the site of Pt binding with cytochrome c has been identified.The results have proved that the OPA precolumn derivatization is a rapid ultrasensitive and reproducible method which has a detection limit of less than 100 fmoles for analysis of amjno acids in peptide hydroIysates. |
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| Keywords: | O-phlhaldjaldehyde precolumn derivatizalion reversed-p-hase high performance liquid chromalography modfed cyiljochrome c fluorescent-label |
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