| 左手螺旋Z-DNA的研究:人工合成的Oligod(G—C)_n在PAGE中的异常泳动行为 |
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| 引用本文： | 黄汝多,黄德民,王振海,梁镇和,汤锦炎,戴艳姿,崔益本,龚立三,刘颂豪.左手螺旋Z-DNA的研究:人工合成的Oligod(G—C)_n在PAGE中的异常泳动行为[J].安徽大学学报(自然科学版),1991(1). |
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| 作者姓名： | 黄汝多 黄德民 王振海 梁镇和 汤锦炎 戴艳姿 崔益本 龚立三 刘颂豪 |
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| 作者单位： | 安徽大学，安徽大学，安徽大学，上海生物化学研究所，上海生物化学研究所，安徽光学精密机械研究所，安徽光学精密机械研究所，华南师范大学，华南师范大学 |
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| 摘 要： | 为研究DNA的B,Z构象的相互转换,我们人工合成了dG-dC交替互补顺序的六聚体与十二聚体,在用PAGE法纯化过程中,发现5',3'-末端为双羟基的寡聚体的泳动速率是d(G-C)_3>d(G-C)_2>d(G-C),5'—末端带磷酸基的相应寡聚体的泳动速率则是:d~*(G-C)>d~*(G-C)_2>d~*(G-C)_3,二者行为正好相反,而5',3'—末端为双羟基的d(G-C)_5与d(G-C)_6和5'—末端带磷酸基的十聚与十二聚体在PAGE中的泳动行为是一致的。由此推测5',3'—末端为双羟基的寡聚体在PAGE中的异常行为的转折点在六聚到十聚之间。
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| 关 键 词： | Oligod(G-C)_n的合成 异常电泳行为 |
Study of the Left-handed DNA: The Unusual Electrophoritic Behaviour of Oligod ( G-C ) Having 5 '-, 3'- Bishydroxy Groups Synthesized by Solid-Phase-Phosphite-Triester Method in Polyacrylamide Gel |
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| Huang Ruduo,Liang Zhenhe,Tang Jinyan Dai Yanzhi,Gong Lisan,Cui Yaeben Huang Demin,Wang Zhenhai,Liu Songhao.Study of the Left-handed DNA: The Unusual Electrophoritic Behaviour of Oligod ( G-C ) Having 5 ''''-, 3''''- Bishydroxy Groups Synthesized by Solid-Phase-Phosphite-Triester Method in Polyacrylamide Gel[J].Journal of Anhui University(Natural Sciences),1991(1). |
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| Authors: | Huang Ruduo Liang Zhenhe Tang Jinyan Dai Yanzhi Gong Lisan Cui Yaeben Huang Demin Wang Zhenhai Liu Songhao |
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| Abstract: | All oligodeoxynucletides were synthesized by Solid-Phase-Phosphite-Triester method, and purified with PAGE. We observed that the electrophoretic mobility was oligod (G-C)_3>d (G-C)_2>d (G-C), just in reverse order with regard to oligomtrs containing terminal phosphate resides. The electrophoritjc behaviour of oligod (G-C)_6,d (G-C)_5carryjng the hydroxy or phosPhated groups at terminal was the same. Hance, we suggest that the turning point was probably locating between 6th mer to 10th mer. |
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| Keywords: | Synthesized Oligod (G-C)_n unusual electrophoritic behaviour |
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