| 蜜环菌胞外多糖的分离纯化及其性质研究 |
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| 引用本文： | 孙玉军,陈彦,王洵,李燕斐.蜜环菌胞外多糖的分离纯化及其性质研究[J].安徽大学学报(自然科学版),2005,29(5):87-91. |
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| 作者姓名： | 孙玉军 陈彦 王洵 李燕斐 |
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| 作者单位： | 安徽大学,生命科学学院,安徽,合肥,230039;安徽技术师范学院,应用生物系,安徽,凤阳,233100;安徽大学,生命科学学院,安徽,合肥,230039 |
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| 基金项目： | 安徽省高等学校自然科学研究基金重点资助项目（2005KJ020ZD） |
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| 摘 要： | 从蜜环菌发酵液提取粗多糖,经分离纯化得到多糖-A和多糖-B两个组分,快速纯化系统(FPLC)和电泳法鉴定各自为均一的组分。FPLC法测定A组分的分子量分别为2.0×105,苯酚-硫酸法测得其总糖含量为86.6%,考马斯亮蓝法测其蛋白含量为12.92%,硫酸-咔唑法测其酸性多糖含量为28%,红外光谱呈现多糖吸收特征峰,含有吡喃糖苷键。β-消去反应初步证明所提取的多糖-A存在O-糖肽键。
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| 关 键 词： | 蜜环菌 胞外多糖 分离纯化 多糖性质 |
| 文章编号： | 1000-2162（2005）05-0087-05 |
| 收稿时间： | 2005-04-05 |
| 修稿时间： | 2005-04-05 |
Studies on the isolation, purifications and characterization of an extracellular polysaccharide from armillaria mellea |
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| SUN Yu-jun,CHEN Yan,WANG Xun,LI Yan-fei.Studies on the isolation, purifications and characterization of an extracellular polysaccharide from armillaria mellea[J].Journal of Anhui University(Natural Sciences),2005,29(5):87-91. |
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| Authors: | SUN Yu-jun CHEN Yan WANG Xun LI Yan-fei |
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| Institution: | SUN Yu-jun~ |
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| Abstract: | A crude polysaccharide could be distilled from Armillaria mellea,after purified by DEAE-Cellulose chromatography,we could get the polysaccharide.Through further purification by Sephacryl S-300 chromatography,we could get polysaccharide-A and polysaccharide-B which were identified to be homogeneous polysaccharide-protein complex by electrophoresis and fast protein liquid chromatography(FPLC).The molecular weight of the polysaccharide-protein A was 2.0 10~(5).The content of total saccharide was 86.6%.The content of total protein was 12.92%.The content of total acid polysaccharide was 28%.The IR of polysaccharide A showed characteristic absorptions of polysaccharide.β-elimination reaction demonstrated that the saccharide were linked to peptides chain through O-glycosidic linkage in it. |
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| Keywords: | Armillaria mellea extracellular Polysaccharide isolation and purification Polysaccharide characterization |
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