党参多糖的研究 Ⅰ党参多糖组分的分离纯化及其部分性质

中药党参的水提液经乙醇沉淀,酶法处理后,经Sevag法脱蛋白,对蒸馏水充分透析,经DEAE-SephadexA-50柱层析,得FP-l和FP-2两个多糖组分,二者产率比为l:3。FP-l和FP-2经醋酸纤维薄膜电泳,纸电泳,玻璃纤维纸高压电泳和凝胶柱层析都证明是一均一组分,经凝胶过滤法测得FP-l和FP-2的分子量分别为50万和2万左右。 FP-l和FP-2的旋光性均为正值,比旋度分别为210°和20.5°用Discher法测试表明,二者均含有糖醛酸。经纸层析,薄板单向层析,双向层析分析,FP-l含鼠李糖,甘露糖、半乳糖和葡萄糖醛酸。FP-2含木糖、半乳糖醛酸和一未知糖。红外光谱,H-核磁共振测定结果表明,FP-1糖苷健构型为β-型,FP-2为α-型。 FP-1和FP-2经高碘酸氧化及Smith降解,表明FP-l以1→3连接键存在,而FP-2除了以l→3为主要连接方式,尚有少量的l→6和1→4键连接的分枝链。

STUDIES ON POLYSACCHARIDES FROM CODONOPSIS PILOSULA

Two Polysaccharides (FP-1 and FP-2)are obtained from Codonopsis posula. Two ratio: 1: 13. M. w. of FP—l and FP-2 are 50×10~4 and 2×10~4. FP-1 land FP-2havea]D+210°and+20.5°. Both Polysaccharides contain uronic acid by Dische method. FP-1 contains rhamnose, mannose, galactose and glucosyl uronic acid, FP-2 contains xylose and galactosyl uronic acid and non-known sugar by means of paper chromatography. I. r. spectra and HNMR indicate that FP-1 has α-glycoside linkage and FP-2 has β-glycoside linkage. From the results of periodate oxidation and Smith degration, itis estimated that FP-1 only has 1→3 linkages and FP-2 has 1→3 linkages in its main chain with 1→6 and 1→4 linkage in its side chain.