| 薄层色谱法测定戊二酰基-7-氨基头孢烷酸酰化酶的活力 |
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| 引用本文： | 姜先荣,杨杰.薄层色谱法测定戊二酰基-7-氨基头孢烷酸酰化酶的活力[J].安徽大学学报(自然科学版),1998,22(2):108-110. |
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| 作者姓名： | 姜先荣 杨杰 |
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| 作者单位： | 安徽教育学院生物系 |
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| 摘 要： | 用薄板层析分离ＧＬ＿７＿ＡＣＡ酰化酶反应的产物和底物，然后用薄层扫描仪进行扫描定量，经过与ＧＬ＿７＿ＡＣＡ和７＿ＡＣＡ标准品的薄层扫描相比较，得出酶反应液中的７＿ＡＣＡ浓度，从而计算出ＧＬ＿７＿ＡＣＡ酰化酶的活力
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| 关 键 词： | GL-7-ACA酰化酶，7-氨基头孢烷酸（7-ACA），戊二酰基-7-氨基头孢烷酸（GL-7-ACA），薄层 |
Determination of Activity of Glutaryl_7_Amidocephalosporanic Acid Acylase by Thin_Layer Chromatography |
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| Jiang Xianrong,Yang Jie,Ren Keqin.Determination of Activity of Glutaryl_7_Amidocephalosporanic Acid Acylase by Thin_Layer Chromatography[J].Journal of Anhui University(Natural Sciences),1998,22(2):108-110. |
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| Authors: | Jiang Xianrong Yang Jie Ren Keqin |
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| Institution: | Department of Biology Anhui Institute of Education Hefei 230061 |
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| Abstract: | The products and substrates of GL_7_ACA acylase were separated by Thin_Layer chromatography and were quantitativly determinated by Thin_Layer scanning. Compared with the Thin_Layer scanning of the standard 7_ACA and GL_7_ACA,the concentration of 7_ACA in the reaction mixture and the GL_7_ACA acylase's activity were calculated. |
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| Keywords: | GL_7_ACA acylase 7_Amidocephalosporanic Acid (7_ACA) Glutaryl_7_Amidocephalosporanic Acid (GL_7_ACA) thin_layer chromatography |
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