毛细管电泳有效分离和同时检测8种氟喹诺酮

对快速分离和同时测定人体尿液中8种氟喹诺酮的毛细管区带电泳新方法进行了研究.采用高效毛细管电泳进行分离;以4×10-2 mol/L硼砂和0.1 mol/L磷酸作电泳缓冲溶液(pH=9.15);5 kPa压力和25℃条件下进样4 s,在22 kV电压下进行毛细管电泳分离,以紫外检测器于278 nm波长下检测.8种氟喹诺酮的校准曲线呈良好的线性关系(r0.999 5),回收率为81.82%～104.24%;检出限为1.05～2.08 mg/L,相对标准偏差小于4%.所提出的方法能有效分离和同时测定人体尿液中的多种喹诺酮.

Effective Separation and Simultaneous Determination of Eight Fluoroquinolones by Capillary Electrophoresis

To develop a new method for effective separation and simultaneous determination of eight fluoroquinolones in human urine by capillary electrophoresis.Sampling for 4 s at 5 kPa and 25 ℃,separation by capillary electrophoretic method at 22 kV using a mixture consisted of 4×10-2 mol/L Na2B4O7 and 0.1 mol/L H3PO4(pH=9.15) as running buffer,detection by using a diode-array detector at wavelength 278 nm.Calibration curves of eight fluoroquinolones were linear with a correlation coefficient greater than 0.999 5.The recoveries were in the range of 81.82% ～ 104.24%.The detection limits of gatifloxacin,lomefloxacin,enoxacin,ciprofloxacin,ofloxacin,enrofloxacin,fleroxacin and pefloxacin were 2.08,1.73,1.29,1.73,1.40,1.14,1.05 and 1.26 mg/L,respectively.The relative standard deviation(RSD) was less than 4%.The proposed method can be applied for effective separation and simultaneous determination of eight fluoroquinolones studied in human urine.