HPLC测定茵栀黄方剂中4味药材的主要成分及含量

建立HPLC检测茵栀黄方剂中4味药材标准提取物化学成分的分析方法,并测定各提取物中绿原酸、1.3-二咖啡酰奎宁酸、异绿原酸B、异绿原酸A、异绿原酸C、栀子苷、黄芩苷、汉黄芩素、黄芩素、汉黄芩苷、千层纸素A及新绿原酸的含量.采用Diamonsil C₁₈(250 mm×4.6 mm, 5 μm)色谱柱,流动相为乙腈(A)-0.1%(体积分数)甲酸水溶液(B),梯度洗脱,流速 1.0 mL/min,柱温30 ℃.在该色谱条件下主要成分能得到较好的分离,稳定性、重复性及精密度良好,平均加样回收率为98.84% ~ 102.22%.该方法能简便有效地测定4味药材标准提取物中多种成分的含量,结果准确可靠,能为茵栀黄复方药材提取物的质量标准制定提供参考,为其主要化学成分分析提供依据.

Analysis and determination of the main components in the four medicinal materials of Yinzhihuang formula by HPLC

The chemical constituents of the standard extracts of the four medicinal materials in Yinzhihuang formula were analyzed by HPLC, and determined the content of chlorogenic acid, 1,3-dicaffeoylquinic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, geniposide, baicalin, wogonin, baicalein, wogonoside, oroxylin A, and neochlorogenic acid. The separation was carried out on a Diamonsil C₁₈(250 mm×4.6 mm,5 μm)column, and gradient eluted with the mobile phase of acetonitrile(A)-0.1% formic acid aqueous solution(B)with 1.0 mL/min. The column temperature was set at 30 ℃. The main components in the four standard medicinal extracts of Yinzhihuang formula can be separated in the chromatographic conditions. Precision, repeatability, and stability were good, and the average recoveries were between 98.84%~102.22%. The developed method was simple and effective for the determination of the components in the four medicinal materials, and the result is accurate and reliable, it can provide a reference for the Yinzhihuang formulation of the quality standard of medicinal extracts and a basis for the analysis of the main chemical components.