紫色红曲霉的微波诱变及其产红曲色素发酵条件的优化

以紫色红曲霉S1为出发菌株进行微波诱变,筛选出产红曲色素能力最高的突变株紫色红曲霉S1-29,其红曲色素值达3 240 U/g,比出发菌株提高了57%.针对菌株S1-29固态发酵培养产红曲色素的条件进行优化,添加不同的碳源、氮源以及无机盐,研究添加物对产红曲色素能力的影响,并进行正交实验,得到最佳发酵条件:在大米基质中添加葡萄糖、蛋白胨、硫酸镁和磷酸氢二钾,使其质量分数分别为4%,6%,0.4%和0.3%,32 ℃培养11 d,菌株S1-29红曲色素值达到4 003 U/g,比优化前提高23.55%.

Microwave mutagenesis of Monascus purpureus and optimization of fermentation conditions for Monascus pigment production

The microwave mutagenesis was carried out when Monascus purpureus S1 was used as the original strain. M.purpureus S1-29 was screened with the highest Monascus pigment value of 3 240 U/g dry base, which was increased by 57% compared with that of the original strain. The conditions for the production of Monascus pigment by solid-state fermentation of strain S1-29 were optimized. The effects of carbon sources, nitrogen sources and inorganic salts on pigment production were investigated and orthogonal experiments were carried out. The results showed that when 4% glucose, 6% peptone, 0.4% magnesium sulfate and 0.3% dipotassium hydrogen phosphate were added into the rice media and cultured at 32 ℃ for 11 days, the Monascus pigment value of M.purpureus S1-29 reached 4 003 U/g dry base, which was increased by 23.55% compared with that of control.