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PGPR芽孢杆菌B-1菌株的鉴定及其应用效果
引用本文:张爱民,张双凤,赵钢勇,张瑞英.PGPR芽孢杆菌B-1菌株的鉴定及其应用效果[J].河北大学学报(自然科学版),2011,31(3):294-298.
作者姓名:张爱民  张双凤  赵钢勇  张瑞英
作者单位:1. 河北大学生物技术研究中心,河北,保定,071002
2. 河北大学生命科学学院,河北,保定,071002
摘    要:从国外产品中分离得到一株PGPR菌株,编号为B-1;观察了该茵株的形态大小和茵落形态,进行了生理生化反应,通过热变性法测定其G+C(鸟嘌呤-胞嘧啶)摩尔分数为47.2%,鉴定该菌株为短芽孢杆菌(Bacillus brevis);通过小麦水培实验研究了该菌株对小麦株高和鲜重的影响,地上部鲜重较对照增加10.64%,株高较...

关 键 词:筛选分离  短芽孢杆菌  小麦水培实验  玉米盆栽实验

Applied Effects and the Identification of PGPR Bacillus B-1 Strain
ZHANG Ai-min,ZHANG Shuang-feng,ZHAO Gang-yong,ZHANG Rui-ying.Applied Effects and the Identification of PGPR Bacillus B-1 Strain[J].Journal of Hebei University (Natural Science Edition),2011,31(3):294-298.
Authors:ZHANG Ai-min  ZHANG Shuang-feng  ZHAO Gang-yong  ZHANG Rui-ying
Institution:1.Center of Biological Technology,Hebei University,Baoding 071002,China;2.College of Life Sciences,Hebei University,Baoding 071002,China)
Abstract:A PGPR strain named B-1 was separated from foreign products,the morphology of the cell and the clony was observed and physiological and biology-chemical reaction were made.DNA G+C mol% was 47.2% by the method of thermal denaturation,so the strain was identified as Bacillus brevis;The height and the fresh weight was studied by the experiments of wheat inoculated with it.The fresh weight of wheat was increased by 10.64% and the plant height was increased by 6.75%.The maize pot experiment results showed that significant increasing effect could be got,plant height and leaf area could be increased than that of the control and Bacillus mucilaginosus.Maize plant height could be increased by 8.0% and the leaf area could be increased by 5.6%.
Keywords:separation  Bacillus brevis  wheat water experiment  maize pot experiment
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