| 维生素B＿2产生菌的原生质体制备及再生 |
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| 引用本文： | 王俊刚,朱宝成,安海谦,李庆余.维生素B＿2产生菌的原生质体制备及再生[J].河北大学学报(自然科学版),1995(1). |
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| 作者姓名： | 王俊刚 朱宝成 安海谦 李庆余 |
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| 作者单位： | 承德医学院生物教研室 |
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| 摘 要： | 用１．０％纤维素酶＋０．５％蜗牛酶的混合酶液酶解培养２６～３２小时的维生素Ｂ＿２产生菌阿氏假囊酵母（Ｅｒｅｍｏｔｈｅｃｉｕｍａｏｈｂｙｉｉ）菌丝体，可以得到大量原生质体，最佳酶解时间为１．５小时。原生质体再生率为０．３５％～０．６７％。阿氏假囊酵母在原生质体再生过程中具有较高的突变率，以维生素Ｂ＿２产量为指标的正突变率为１２．１４％。
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| 关 键 词： | 阿氏假囊酵母，原生质体，再生 |
Preparation and Regeneration of Protoplasts from Vitamin B_2 Producer Eremothecium aohbyii |
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| Wang Jungang Zhu Baocheng An Haiqian Li Qingyu.Preparation and Regeneration of Protoplasts from Vitamin B_2 Producer Eremothecium aohbyii[J].Journal of Hebei University (Natural Science Edition),1995(1). |
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| Authors: | Wang Jungang Zhu Baocheng An Haiqian Li Qingyu |
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| Abstract: | Using a combined enzyme system containing 1.0% cellulase(ONOZUKAR-10)plus 0.5 % snail digestase,high yield of protoplasts from Eremothecium aohbyiimycelia which had grown for 26-32 hours was obtained.The regeneration frequency were0.35%-0.67%. Many mutants appeared in the regeneration strains on solid medium. Posi-tive mutation ratio was 12.14% by determining the yield of Vitamin B_2. |
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| Keywords: | Eremothecium aohbyii Protoplast Regeneration |
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