| 人组氨酰-tRNA合成酶基因的克隆与表达 |
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| 引用本文： | 毕智丽,吴亦红,赵晓瑜,倪志华,王三元.人组氨酰-tRNA合成酶基因的克隆与表达[J].河北大学学报(自然科学版),2005,25(3):318-322. |
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| 作者姓名： | 毕智丽 吴亦红 赵晓瑜 倪志华 王三元 |
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| 作者单位： | 1. 河北大学,生命科学学院,河北,保定,071002
2. 河北省环境科学研究院,河北,石家庄,050051 |
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| 摘 要： | 用RT_PCR方法从人胎盘总RNA中获得编码人组氨酰_tRNA合成酶(Jo_1)基因的全序列,选用IMPACT_CN系统中的pTYB11,构建克隆与表达载体,转化大肠杆菌ER2566.经筛选与鉴定,得到3个阳性重组子,对其进行诱导表达获得Jo_1融合蛋白,western_blotting检验结果显示,该融合蛋白具有Jo_1免疫原性和免疫特异性.
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| 关 键 词： | 人组氨酰-tRNA合成酶 基因克隆 表达 自身抗原 |
| 文章编号： | 1000-1565(2005)03-0318-05 |
| 修稿时间： | 2004年10月20 |
Cloning and Expression of Human Histidyl-tRNA Synthetase |
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| BI Zhi-li,WU Yi-hong,ZHAO Xiao-Yu,NI Zhi-hua,WANG San-yuan.Cloning and Expression of Human Histidyl-tRNA Synthetase[J].Journal of Hebei University (Natural Science Edition),2005,25(3):318-322. |
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| Authors: | BI Zhi-li WU Yi-hong ZHAO Xiao-Yu NI Zhi-hua WANG San-yuan |
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| Institution: | BI Zhi-li1,WU Yi-hong2,ZHAO Xiao-yu1,NI Zhi-hua1,WANG San-yuan1 |
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| Abstract: | Using RT-PCR technology the full length DNA sequence coding for human histidyl-tRNA synthetase was obtained. The pTYB11 was used in construction of expressing vector. The recombinant plasmid constructed was transformed into ER2566 and the system was used to express fusion Jo-1 antigen. At last, the author got 3 recombinants that could successfully express the fusion protein. Meanwhile, the expressed fusion protein could be identified by western-blotting using anti-Jo-1 antibody. |
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| Keywords: | human histidyl-tRNA synthetase gene cloning expression autoantigen |
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