| Luminol-H2O2化学发光体系检测铁蛋白 |
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| 引用本文： | 李正平,韩军,吕学冲,刘秀贤.Luminol-H2O2化学发光体系检测铁蛋白[J].河北大学学报(自然科学版),2009,29(3):274. |
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| 作者姓名： | 李正平 韩军 吕学冲 刘秀贤 |
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| 作者单位： | 河北大学,化学与环境科学学院,河北,保定071002 |
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| 摘 要： | 酸性介质中, 铁蛋白(Ferritin)催化luminol-H2O2反应并产生很强的化学发光(CL)信号.基于此, 建立了简便灵敏的化学发光检测铁蛋白的分析方法,其线性范围为0.5～10 μg/L, 检出限(3σ)为0.36 μg/L,为铁蛋白作为纳米粒子标记物并直接检测提供了一种新的途径.
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| 关 键 词： | 化学发光 铁蛋白 鲁米诺 |
Determination of Ferritin with Luminol-H2O2 Chemiluminescence System |
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| LI Zheng-ping,HAN Jun,LU Xue-chong,LIU Xiu-xian.Determination of Ferritin with Luminol-H2O2 Chemiluminescence System[J].Journal of Hebei University (Natural Science Edition),2009,29(3):274. |
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| Authors: | LI Zheng-ping HAN Jun LU Xue-chong LIU Xiu-xian |
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| Abstract: | In acidic medium,Ferritin can catalyze luminol-H2O2 chemiluminescence(CL) reaction and produce strong CL signal.Based on this phenomenon,a simple and sensitive method for Ferritin determination has been developed.There was a good linear relationship between the CL intensity and the concentration of Ferritin in the range of 0.5～10 μg/L,and the detection limit(3σ) was 0.36 μg/L.The proposed method offers a new approach for direct detection of Ferritin as labeled nanoparticles. |
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| Keywords: | chemiluminescence ferritin luminol |
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