| 马铃薯StHb1蛋白的亚细胞定位 |
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| 引用本文： | 李继刚,郑建坡,曲占良.马铃薯StHb1蛋白的亚细胞定位[J].河北大学学报(自然科学版),2012,32(5):523-527. |
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| 作者姓名： | 李继刚 郑建坡 曲占良 |
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| 作者单位： | 河北大学 生命科学学院,河北省微生物多样性研究与应用重点实验室,河北保定071002 |
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| 基金项目： | 河北大学博士基金资助项目(2007-097);河北省教育厅资助项目(2007412);河北省科技支撑计划项目(11215529) |
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| 摘 要： | 为了研究马铃薯StHb1蛋白的亚细胞定位情况,采用RT-PCR技术克隆到马铃薯StHb1基因cDNA序列,并成功构建了StHb1基因与绿色荧光蛋白基因的融合表达载体pBI121-StHb1-GFP.利用农杆菌介导法将重组载体转化洋葱内表皮细胞,通过荧光显微镜观察融合蛋白的瞬时表达以确定StHb1蛋白在细胞内的分布.结果表明StHb1蛋白主要分布于细胞核中.
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| 关 键 词： | 非共生血红蛋白 绿色荧光蛋白(GFP) 亚细胞定位 融合表达载体 农杆菌介导转化 |
Subcellular localization of StHb1 protein from potato |
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| LI Jigang
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ZHENG Jianpo
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QU Zhanliang.Subcellular localization of StHb1 protein from potato[J].Journal of Hebei University (Natural Science Edition),2012,32(5):523-527. |
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| Authors: | LI Jigang ZHENG Jianpo QU Zhanliang |
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| Institution: | (Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences,Hebei University,Baoding 071002,China) |
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| Abstract: | To identify the subcellular location of StHb1 protein,a cDNA encoding nonsymbiotic hemoglobin(StHb1) was cloned from Solanum tuberosum by RT-PCR,and fused to the 5′ end of the green fluorescent protein gene to generate plant binary vector pBI121-StHb1-GFP.It was introduced into onion epidermal cells via Agrobacterium-mediated transformation.Subcellular localization of StHb1 protein was detected by fluorescent microscopy.The results showed that the StHb1-GFP fusion proteins were predominantly present in the nucleus. |
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| Keywords: | non-symbiotic hemoglobin green fluorescent protein(GFP) subcellular localization fusion expression vector Agrobacterium-mediated transformation |
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