灵芝营养液的发酵法制备及分析

对灵芝营养液的制备条件进行了探讨。研究表明，筛选所获灵芝菌株在（２９±１）℃，ｐＨ４．０～５．０的适宜条件下，经９６ｈ深层通气培养可获得菌丝体鲜重＞３５０ｇ／Ｌ的发酵醪液。该研究采用的培养基适用性广，发酵醪液即可应用于营养液的制备。细胞破壁处理可有效地提高提取液中多糖及氨基酸的质量浓度，经低温浓缩后的灵芝营养液氨基酸构成全面，其质量浓度为１１．１２ｇ／Ｌ，氨基酸中必需氨基酸的质量分数为３２％；多糖的质量浓度为１２ｇ／Ｌ；还测得有核苷类、甾醇类物质。该营养液外观黑红色、不透明，重金属、大肠杆菌及其它致病菌等理化卫生指标符合国家有关规定。急性毒性实验表明受试物安全无毒（ＬＤ５０＞２１．５ｇ／ｋｇ）。经小鼠游泳耗竭实验证明灵芝营养液具有抗疲劳作用。

The Preparation and Analysis of the Nutrient Solution of Ganoderma lucidum by Fermentation

Studies on submerged culture of Ganoderma lucidum show that the optimal condition is (29±1) ℃ and pH 4.0～5.0. After 96 h culture in fermenter the biomass amounts up to 350 g/L(fresh weight). The nutrient solution can be prepared by extracting of cells and concentrating at low temperature, and this treatment in turn effectuvely increase production of amino acids and polysaccharide in the nutrient solution, which is 11.12 g/L and 12 g/L respectively. The nutrient solution contains complete composition of amino acids, also some nucleotides and sterols, and the amount of essential amino acids is 32%. The solution is dark red and opaque. The amount of heavy metals, E.coli and other pathogenic bacteria are less than national standard. It is safe according to the acute toxic test (LD50>21.5 g/kg). Rat swimming tests show that the nutrient solution can relieve fatigue markedly.