| 胭脂花的组织培养与植株再生 |
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| 引用本文： | 宋韵霏,王俊丽,毕凯丽,张璐,李建飞.胭脂花的组织培养与植株再生[J].河北大学学报(自然科学版),2012,32(6):630-634. |
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| 作者姓名： | 宋韵霏 王俊丽 毕凯丽 张璐 李建飞 |
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| 作者单位： | 中央民族大学生命与环境科学学院,北京,100081 |
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| 基金项目： | "973"计划资助项目，中央高校基本科研业务费专项资金资助项目，国家民委资助项目，985工程资助项目，高等学校学科创新引智计划资助项目 |
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| 摘 要： | 为了有效保护和利用野生胭脂花资源,以胭脂花(Primula maximowiczii)叶片、叶柄作为外植体进行组织培养及植株再生研究,建立了胭脂花的离体无性繁殖体系.结果表明,叶柄不定芽诱导率较高.经实验筛选出最适不定芽诱导培养基为MS+2,4-D 1.0mg/L+TDZ 2.0mg/L,最佳不定芽增殖培养基为MS+2,4-D 4.0mg/L+6-BA 2.0mg/L,幼苗移栽成活率可达60%以上.
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| 关 键 词： | 胭脂花 组织培养 植株再生 |
Tissue culture and plant regeneration of Primula maximowiczii |
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| SONG Yunfei
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WANG Junli
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BI Kaili
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ZHANG Lu
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LI Jianfei.Tissue culture and plant regeneration of Primula maximowiczii[J].Journal of Hebei University (Natural Science Edition),2012,32(6):630-634. |
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| Authors: | SONG Yunfei WANG Junli BI Kaili ZHANG Lu LI Jianfei |
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| Institution: | (College of Life and Environmental Sciences,Minzu University of China,Beijing 100081,China) |
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| Abstract: | A propagation system of Primula maximowiczii in vitro was established by using leaf explants and petiole explants.The results showed that petiole explants has a higher adventitious shoot induction rate.The optimal medium for inducing adventitious shoots from petiole explants was MS+2,4-D 1.0 mg/L+TDZ 2.0 mg/L and the best medium for subculture was MS+2,4-D 4.0 mg/L+6-BA 2.0 mg/L.The survival rate for regenerated plants was about 60%. |
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| Keywords: | Primula maximowiczii tissue culture plant regeneration |
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