| 关于产生细胞分裂素的紫云英根瘤菌突变株发酵条件的探索 |
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| 引用本文： | 卢绍杰,梁伯璠.关于产生细胞分裂素的紫云英根瘤菌突变株发酵条件的探索[J].河北大学学报(自然科学版),1990(4). |
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| 作者姓名： | 卢绍杰 梁伯璠 |
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| 作者单位： | 天津医学院
(卢绍杰)，河北大学生物系(梁伯璠) |
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| 摘 要： | 紫云英根瘤菌Rhizobium sp(Atragalus)突变株在修改后的化学合成培养基和液态常规培养基上都能正常生长,而且分泌细胞分裂素的量都是在菌体生长的稳定期为最大。但以化学合成培养基为更适宜。菌体在其上生长,产生的细胞分裂素更多。培养基的碳源以10g/l甘露醇为宜。氮源以0.5g/l NH_4NO_3和0.75g/l尿素为宜。
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| 关 键 词： | 突变株 碳源 氮源 |
Studies on Fermentative Condition for Mutant 96 Producing Cytokinin |
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| Lu shaojie Liang Bo fan.Studies on Fermentative Condition for Mutant 96 Producing Cytokinin[J].Journal of Hebei University (Natural Science Edition),1990(4). |
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| Authors: | Lu shaojie Liang Bo fan |
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| Institution: | Lu shaojie Liang Bo fan
(Tianjin Medical College) (Department of Biology) |
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| Abstract: | Mutant 96 was able to growth on altered Chemical synthetic or Liguid common Culture normaly. Period of highest amount producing Cytokinin by mutant 96 was in proto-stable phase. Chemical Synthetic culture was suitable to mutant 96 because it has produced more cytokinin on this Culture. It was suitable that we took 10g/1 mannitol as Carbon Source and 0.5g /1NH4NO3 or 0.75g /1 urea as nitrogen source. |
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| Keywords: | Mutant Carbon Source Nitrogen Source |
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